布氏锥虫60S核糖体蛋白L27a（L29）基因分析

Analysis of the 60 S ribosomal protein L27a (L29) gene of Trypanosoma brucei.

作者信息

Brown S V, Williams N

机构信息

Department of Microbiology, State University of New York at Buffalo 14214, USA.

出版信息

Int J Parasitol. 1999 May;29(5):731-6. doi: 10.1016/s0020-7519(99)00023-5.

DOI:10.1016/s0020-7519(99)00023-5

PMID:10404268

Abstract

The Trypanosoma brucei gene encoding the 60 S ribosomal protein L27a (L29) homologue has been cloned and characterised. The complete open reading frame encodes a small basic protein of 145 amino acids with a predicted molecular weight of 15,950. The L27a amino acid sequence shares 45-58% identity with other L27a (L29) homologues. Southern blot hybridisation suggests that the gene is present in multiple copies. Northern blot analysis of RNA from three T. brucei life cycle stages show that mRNA levels are two-fold higher in procyclic than in early or late bloodstream stages. This infers that this highly conserved ribosomal protein may play an important role in translational regulation through the life cycle of trypanosomes.

摘要

编码60 S核糖体蛋白L27a（L29）同源物的布氏锥虫基因已被克隆并鉴定。完整的开放阅读框编码一个由145个氨基酸组成的小碱性蛋白，预测分子量为15,950。L27a氨基酸序列与其他L27a（L29）同源物的同一性为45 - 58%。Southern印迹杂交表明该基因以多拷贝形式存在。对布氏锥虫三个生命周期阶段的RNA进行Northern印迹分析显示，前循环期的mRNA水平比早期或晚期血流期高两倍。这表明这种高度保守的核糖体蛋白可能在锥虫的整个生命周期的翻译调控中发挥重要作用。