起始因子sigma(70)与核心RNA聚合酶的结合是一个多步骤过程。

Binding of the initiation factor sigma(70) to core RNA polymerase is a multistep process.

作者信息

Gruber T M, Markov D, Sharp M M, Young B A, Lu C Z, Zhong H J, Artsimovitch I, Geszvain K M, Arthur T M, Burgess R R, Landick R, Severinov K, Gross C A

机构信息

Departments of Stomatology and Microbiology and Immunology, University of California, San Francisco 94143, USA.

出版信息

Mol Cell. 2001 Jul;8(1):21-31. doi: 10.1016/s1097-2765(01)00292-1.

DOI:10.1016/s1097-2765(01)00292-1

PMID:11511357

Abstract

The interaction of RNA polymerase and its initiation factors is central to the process of transcription initiation. To dissect the role of this interface, we undertook the identification of the contact sites between RNA polymerase and sigma(70), the Escherichia coli initiation factor. We identified nine mutationally verified interaction sites between sigma(70) and specific domains of RNA polymerase and provide evidence that sigma(70) and RNA polymerase interact in at least a two-step process. We propose that a cycle of changes in the interface of sigma(70) with core RNA polymerase is associated with progression through the process of transcription initiation.

摘要

RNA聚合酶与其起始因子的相互作用是转录起始过程的核心。为了剖析该界面的作用，我们着手鉴定RNA聚合酶与大肠杆菌起始因子sigma(70)之间的接触位点。我们鉴定出了sigma(70)与RNA聚合酶特定结构域之间9个经突变验证的相互作用位点，并提供证据表明sigma(70)与RNA聚合酶至少通过两步过程相互作用。我们提出，sigma(70)与核心RNA聚合酶界面的一系列变化与转录起始过程的进展相关。