科威特儿童隐孢子虫病的独特地方性流行情况。
Unique endemicity of cryptosporidiosis in children in Kuwait.
作者信息
Sulaiman Irshad M, Hira Parsotam R, Zhou Ling, Al-Ali Faiza M, Al-Shelahi Fatima A, Shweiki Hussein M, Iqbal Jamshaid, Khalid Nabila, Xiao Lihua
机构信息
Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Highway, Atlanta, GA 30341-3717, USA.
出版信息
J Clin Microbiol. 2005 Jun;43(6):2805-9. doi: 10.1128/JCM.43.6.2805-2809.2005.
To understand the transmission of Cryptosporidium infection in children, fecal specimens from 62 Kuwaiti children with gastrointestinal symptoms found to be positive by microscopy were genotyped and subtyped with a small subunit rRNA-based PCR-restriction fragment length polymorphism analysis and a 60-kDa glycoprotein-based DNA sequencing tool. The median age of infected children was 4.5 years, and 77% of infections occurred during the cool season of November to April. Fifty-eight of the children (94%) had Cryptosporidium parvum, three (5%) had Cryptosporidium hominis, and one (1%) had both C. parvum and C. hominis. Altogether, 13 subtypes of C. parvum (belonging to four subtype allele families) and C. hominis (belonging to three subtype allele families) were observed, with 92% of specimens belonging to the common allele family IIa and the unusual allele family IId. Thus, the transmission of cryptosporidiosis in Kuwaiti children differed significantly from other tropical countries.
为了解隐孢子虫感染在儿童中的传播情况，对62名有胃肠道症状且经显微镜检查发现呈阳性的科威特儿童的粪便标本进行基因分型和亚型分析，采用基于小亚基核糖体RNA的聚合酶链反应-限制性片段长度多态性分析和基于60 kDa糖蛋白的DNA测序工具。受感染儿童的中位年龄为4.5岁，77%的感染发生在11月至4月的凉爽季节。58名儿童（94%）感染微小隐孢子虫，3名（5%）感染人隐孢子虫，1名（1%）同时感染微小隐孢子虫和人隐孢子虫。共观察到13种微小隐孢子虫亚型（属于4个亚型等位基因家族）和人隐孢子虫亚型（属于3个亚型等位基因家族），92%的标本属于常见的等位基因家族IIa和不常见的等位基因家族IId。因此，科威特儿童隐孢子虫病的传播与其他热带国家有显著差异。
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