树突状细胞与细胞因子诱导的杀伤细胞共培养对耐药K562细胞细胞毒性的影响

[Effect of dendritic cells co-cultured with cytokine induced killer cells on cytotoxicity against drug resistant K562 cells].

作者信息

Chen Bao-an, Li Man, Sun Zai-yang, Li Cui-ping, Gao Chong, Sun Yun-yu, Wang Jun, Fu Qiang, Chen Jin

机构信息

The Affiliated Zhongda Hospital of Southeast University, Nanjing 210009, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2005 Jun;26(6):355-8.

PMID:16185482

Abstract

OBJECTIVE

To study the effect of dendritic cells (DC) co-cultured with cytokine induced killer (CIK) cells on cytotoxicity against K562 and K562 drug-resistant (K562/ADM) cells.

METHODS

Peripheral blood mononuclear cells (MNC) isolated from healthy adult donors were induced to obtain CIK cells and DC respectively and then these two kinds of cells were co-cultured. The cytotoxicity of the co-cultured cells against K562 and K562/ADM cells was measured with MTT assay.

RESULTS

The cytotoxicity CIK cells alone to K562 and K562/ADM cells was (20.0 +/- 1.2)% - (61.1 +/- 2.2)% and (17.5 +/- 2.1)% - (45.2 +/- 3.3)% respectively at low effector to target ratios (2.5 - 20.0). This effect was significantly enhanced by co-culturing with DCs being (25.2 +/- 2.3)% - (70.9 +/- 4.1)% and (22.4 +/- 2.7)% - (62.3 +/- 5.0)%.

CONCLUSION

CIK cells showed high cytotoxicity against K562 and K562/ADM cells and the activity could be enhanced by co-culturing with DC.

摘要

目的

研究树突状细胞（DC）与细胞因子诱导的杀伤细胞（CIK）共培养对K562及K562耐药（K562/ADM）细胞的细胞毒性作用。

方法

从健康成年供者分离外周血单个核细胞（MNC），分别诱导获得CIK细胞和DC，然后将这两种细胞进行共培养。采用MTT法检测共培养细胞对K562及K562/ADM细胞的细胞毒性。

结果

在低效应细胞与靶细胞比例（2.5 - 20.0）时，单独CIK细胞对K562及K562/ADM细胞的细胞毒性分别为（20.0±1.2）% - （61.1±2.2）%和（17.5±2.1）% - （45.2±3.3）%。与DC共培养后，该效应显著增强，分别为（25.2±2.3）% - （70.9±4.1）%和（22.4±2.7）% - （62.3±5.0）%。