血浆肽基甘氨酸α-酰胺化单加氧酶（PAM）和铜蓝蛋白受大鼠和小鼠年龄及铜状态的影响。
Plasma peptidylglycine alpha-amidating monooxygenase (PAM) and ceruloplasmin are affected by age and copper status in rats and mice.
作者信息
Prohaska Joseph R, Broderius Margaret
机构信息
Department of Biochemistry and Molecular Biology, University of Minnesota Medical School Duluth, 1035 University Drive, 55812, USA.
出版信息
Comp Biochem Physiol B Biochem Mol Biol. 2006 Mar;143(3):360-6. doi: 10.1016/j.cbpb.2005.12.010. Epub 2006 Jan 31.
In an attempt to identify a sensitive and improved marker of mammalian copper status during neonatal development experiments compared two plasma cuproenzymes, peptidylglycine alpha-amidating monooxygenase (PAM ), an enzyme involved in peptide posttranslational activation, to ceruloplasmin (Cp), a ferroxidase involved in iron mobilization. Dietary Cu deficiency (Cu-) was studied in dams and offspring at postnatal age 3 (P3), P12, and P28. Rodent Cp activity rose during lactation whereas PAM activity fell. Reduction in Cp activity was more severe than reduction in PAM activity in Cu- offspring and dams. Cp activity was greater in rats than mice whereas PAM activity was similar in adults but greater in mouse than rat pups. Both cuproenzymes changed during neonatal development and when dietary copper was limiting. With proper controls, each enzyme can be used to assess copper status.
在一项旨在确定哺乳动物新生发育期间铜状态敏感且改良标志物的实验中，研究人员比较了两种血浆铜酶：肽基甘氨酸α-酰胺化单加氧酶（PAM），一种参与肽翻译后激活的酶，和铜蓝蛋白（Cp），一种参与铁转运的铁氧化酶。在产后3天（P3）、12天（P12）和28天（P28）对母鼠及其后代进行了饮食性铜缺乏（Cu-）研究。啮齿动物的Cp活性在哺乳期升高，而PAM活性下降。在铜缺乏的后代和母鼠中，Cp活性的降低比PAM活性的降低更严重。大鼠的Cp活性高于小鼠，而成年动物的PAM活性相似，但幼鼠的PAM活性小鼠高于大鼠。两种铜酶在新生发育期间以及饮食性铜受限的情况下都会发生变化。在适当的对照下，每种酶都可用于评估铜状态。
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