利用慢病毒RNAi文库进行全基因组功能丧失筛选。

Genome-scale loss-of-function screening with a lentiviral RNAi library.

作者信息

Root David E, Hacohen Nir, Hahn William C, Lander Eric S, Sabatini David M

机构信息

Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.

出版信息

Nat Methods. 2006 Sep;3(9):715-9. doi: 10.1038/nmeth924.

DOI:10.1038/nmeth924

PMID:16929317

Abstract

The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.

摘要

RNA干扰（RNAi）在哺乳动物细胞中具有功能这一发现促使我们成立了RNA干扰联盟（TRC），其目标是通过开发基因组规模的RNAi文库及其使用方法来实现大规模功能丧失筛选。这些资源构成了布罗德研究所创建的功能丧失筛选平台的基础。我们的人类和小鼠文库目前包含针对27000个基因的超过135000个慢病毒克隆。初步筛选工作表明，这些文库和方法是用于高通量慢病毒RNAi筛选的实用且强大的工具。