通过荧光原位杂交技术进行高通量端粒长度定量及其在人类群体研究中的应用。
High-throughput telomere length quantification by FISH and its application to human population studies.
作者信息
Canela Andrés, Vera Elsa, Klatt Peter, Blasco María A
机构信息
Telomeres and Telomerase Group, Molecular Oncology Program, Spanish National Cancer Centre, 3 Melchor Fernández Almagro, Madrid E-28029, Spain.
出版信息
Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5300-5. doi: 10.1073/pnas.0609367104. Epub 2007 Mar 16.
Abstract
A major limitation of studies of the relevance of telomere length to cancer and age-related diseases in human populations and to the development of telomere-based therapies has been the lack of suitable high-throughput (HT) assays to measure telomere length. We have developed an automated HT quantitative telomere FISH platform, HT quantitative FISH (Q-FISH), which allows the quantification of telomere length as well as percentage of short telomeres in large human sample sets. We show here that this technique provides the accuracy and sensitivity to uncover associations between telomere length and human disease.
摘要
在人类群体中，研究端粒长度与癌症及年龄相关疾病的相关性以及基于端粒的治疗方法的开发，一个主要限制是缺乏合适的高通量（HT）检测方法来测量端粒长度。我们开发了一种自动化的高通量定量端粒荧光原位杂交平台，即高通量定量荧光原位杂交（Q-FISH），它能够在大量人类样本集中对端粒长度以及短端粒的百分比进行定量分析。我们在此表明，该技术具备揭示端粒长度与人类疾病之间关联的准确性和灵敏度。
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