硒和抗坏血酸对豚鼠组织中维生素C和E的保护作用。

Sparing effects of selenium and ascorbic acid on vitamin C and E in guinea pig tissues.

作者信息

Bertinato Jesse, Hidiroglou Nick, Peace Robert, Cockell Kevin A, Trick Keith D, Jee Penny, Giroux Alex, Madère Réné, Bonacci Giuseppe, Iskandar Monica, Hayward Stephen, Giles Nicholas, L'Abbé Mary R

机构信息

Nutrition Research Division, Food Directorate, Health Products and Food Branch, Health Canada, Sir Frederick G, Banting Research Centre, Ottawa, ON, Canada.

出版信息

Nutr J. 2007 Mar 26;6:7. doi: 10.1186/1475-2891-6-7.

DOI:10.1186/1475-2891-6-7

PMID:17386096

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1847450/

Abstract

BACKGROUND

Selenium (Se), vitamin C and vitamin E function as antioxidants within the body. In this study, we investigated the effects of reduced dietary Se and L-ascorbic acid (AA) on vitamin C and alpha-tocopherol (AT) status in guinea pig tissues.

METHODS

Male Hartley guinea pigs were orally dosed with a marginal amount of AA and fed a diet deficient (Se-D/MC), marginal (Se-M/MC) or normal (Se-N/MC) in Se. An additional diet group (Se-N/NC) was fed normal Se and dosed with a normal amount of AA. Guinea pigs were killed after 5 or 12 weeks on the experimental diets at 24 and 48 hours post AA dosing.

RESULTS

Liver Se-dependent glutathione peroxidase activity was decreased (P < 0.05) in guinea pigs fed Se or AA restricted diets. Plasma total glutathione concentrations were unaffected (P > 0.05) by reduction in dietary Se or AA. All tissues examined showed a decrease (P < 0.05) in AA content in Se-N/MC compared to Se-N/NC guinea pigs. Kidney, testis, muscle and spleen showed a decreasing trend (P < 0.05) in AA content with decreasing Se in the diet. Dehydroascorbic acid concentrations were decreased (P < 0.05) in several tissues with reduction in dietary Se (heart and spleen) or AA (liver, heart, kidney, muscle and spleen). At week 12, combined dietary restriction of Se and AA decreased AT concentrations in most tissues. In addition, restriction of Se (liver, heart and spleen) and AA (liver, kidney and spleen) separately also reduced AT in tissues.

CONCLUSION

Together, these data demonstrate sparing effects of Se and AA on vitamin C and AT in guinea pig tissues.

摘要

背景

硒（Se）、维生素C和维生素E在体内发挥抗氧化剂的作用。在本研究中，我们调查了减少膳食硒和L-抗坏血酸（AA）对豚鼠组织中维生素C和α-生育酚（AT）状态的影响。

方法

给雄性哈特利豚鼠口服少量AA，并饲喂缺硒（Se-D/MC）、边缘性缺硒（Se-M/MC）或正常硒（Se-N/MC）的饮食。另一个饮食组（Se-N/NC）饲喂正常硒并给予正常量的AA。在实验饮食喂养5周或12周后，于AA给药后24小时和48小时处死豚鼠。

结果

饲喂硒或AA受限饮食的豚鼠肝脏中硒依赖性谷胱甘肽过氧化物酶活性降低（P<0.05）。膳食硒或AA的减少对血浆总谷胱甘肽浓度无影响（P>0.05）。与Se-N/NC豚鼠相比，所有检测组织中Se-N/MC豚鼠的AA含量均降低（P<0.05）。随着饮食中硒含量的降低，肾脏、睾丸、肌肉和脾脏的AA含量呈下降趋势（P<0.05）。随着膳食硒（心脏和脾脏）或AA（肝脏、心脏、肾脏、肌肉和脾脏）的减少，几个组织中的脱氢抗坏血酸浓度降低（P<0.05）。在第12周时，联合限制膳食硒和AA会降低大多数组织中的AT浓度。此外，分别限制硒（肝脏、心脏和脾脏）和AA（肝脏、肾脏和脾脏）也会降低组织中的AT。