植物赖氨酰tRNA合成酶表达的改变促进了tRNA的错误酰化以及赖氨酸的翻译重编码。
Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine.
作者信息
Wu Xing Rong, Kenzior Alexander, Willmot David, Scanlon Sarah, Chen Zhihong, Topin Andrey, He Shao Hua, Acevedo Ashley, Folk William R
机构信息
Department of Biochemistry, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA.
出版信息
Plant J. 2007 May;50(4):627-36. doi: 10.1111/j.1365-313X.2007.03076.x. Epub 2007 Apr 8.
The Arabidopsis thaliana lysyl tRNA synthetase (AtKRS) structurally and functionally resembles the well-characterized prokaryotic class IIb KRS, including the propensity to aminoacylate tRNA(Lys) with suboptimal identity elements, as well as non-cognate tRNAs. Transient expression of AtKRS in carrot cells promotes aminoacylation of such tRNAs in vivo and translational recoding of lysine at nonsense codons. Stable expression of AtKRS in Zea mays causes translational recoding of lysine into zeins, significantly enriching the lysine content of grain.
拟南芥赖氨酰tRNA合成酶（AtKRS）在结构和功能上类似于已得到充分表征的原核IIb类KRS，包括用次优识别元件以及非同源tRNA对tRNA(Lys)进行氨酰化的倾向。AtKRS在胡萝卜细胞中的瞬时表达促进了此类tRNA在体内的氨酰化以及无义密码子处赖氨酸的翻译重编码。AtKRS在玉米中的稳定表达导致赖氨酸向醇溶蛋白的翻译重编码，显著提高了籽粒中的赖氨酸含量。
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