来自塞阿拉苦配巴（Copaifera cearensis Huber ex Ducke）、网状苦配巴（Copaifera reticulata Ducke）和多果苦配巴（Copaifera multijuga Hayne）的苦配巴油的化学成分及抗炎活性——一项比较研究

Chemical composition and anti-inflammatory activity of copaiba oils from Copaifera cearensis Huber ex Ducke, Copaifera reticulata Ducke and Copaifera multijuga Hayne--a comparative study.

作者信息

Veiga Junior V F, Rosas E C, Carvalho M V, Henriques M G M O, Pinto Angelo C

机构信息

Departamento de Química, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Av. Gal. Rodrigo Octávio Jordão Ramos 3000, Japiim, 69077-000 Manaus, Brazil.

出版信息

J Ethnopharmacol. 2007 Jun 13;112(2):248-54. doi: 10.1016/j.jep.2007.03.005. Epub 2007 Mar 7.

DOI:10.1016/j.jep.2007.03.005

PMID:17446019

Abstract

Copaiba oil is an oleoresin obtained from the Copaifera L. genus (Leguminoseae) commonly featured in anti-inflammatory recipe prescribed by Amazonian traditional medical practitioners and featured in Europe and North America pharmacopeias of the past. Chemical and anti-inflammatory activity investigations from the copaiba oils obtained from Copaifera multijuga Hayne, Copaifera cearensis Huber ex Ducke and Copaifera reticulata Ducke species have proved that, although similar, these oleoresins possess varied composition and anti-inflammatory activity. Chromatographic studies showed that the main compound among sesquiterpenes was beta-caryophyllene (57.5, 19.7 and 40.9%, respectively), followed by alpha-humulene, alpha-copaene, alpha-bergamotene, delta-cadinene, with different amounts in each oleoresin. Among the diterpenes, copalic acid was the main component from Copaifera multijuga Hayne (6.2%) and was found in all the oleoresins studied. In Copaifera cearensis Huber ex Ducke, clorechinic (11.3%) and hardwickiic acids (6.2%) were the major diterpenes while kaurenoic (3.9%) and kolavenic acids (3.4%) predominated in Copaifera reticulata Ducke. The pharmacologic effects of the three oleoresins were evaluated in vitro by measuring the NO production by murine macrophages and in vivo using the zymosan induced pleurisy model in mice. The Copaiba Oil from Copaifera multijuga Hayne (100 mg/kg) was the most potent, inhibiting both NO production and the pleurisy induced by zymosan. The oleoresins from Copaifera cearensis Huber ex Ducke and Copaifera reticulata Ducke were also able to inhibit NO production and the pleurisy but with less intensity.

摘要

苦配巴油是一种从苦配巴属（豆科）植物中提取的油树脂，常用于亚马逊传统医学从业者开具的抗炎药方中，过去也曾被收录在欧洲和北美的药典中。对多花苦配巴（Copaifera multijuga Hayne）、塞阿拉苦配巴（Copaifera cearensis Huber ex Ducke）和网状苦配巴（Copaifera reticulata Ducke）这三种植物提取的苦配巴油进行的化学和抗炎活性研究表明，尽管这些油树脂相似，但它们的成分和抗炎活性各不相同。色谱研究表明，倍半萜类化合物中的主要成分是β-石竹烯（分别为57.5%、19.7%和40.9%），其次是α-葎草烯、α-古巴烯、α-佛手柑油烯、δ-杜松烯，每种油树脂中的含量不同。在二萜类化合物中，苦配巴酸是多花苦配巴油中的主要成分（6.2%），在所研究的所有油树脂中均有发现。在塞阿拉苦配巴中，氯瑞契宁酸（11.3%）和哈德威克酸（6.2%）是主要的二萜类化合物，而在网状苦配巴中，贝壳杉烯酸（3.9%）和柯拉韦尼酸（3.4%）占主导地位。通过测量小鼠巨噬细胞产生的一氧化氮（NO），在体外对这三种油树脂的药理作用进行了评估，并在体内使用酵母聚糖诱导的小鼠胸膜炎模型进行了评估。多花苦配巴油（100毫克/千克）的效果最为显著，既能抑制NO的产生，又能抑制酵母聚糖诱导的胸膜炎。塞阿拉苦配巴和网状苦配巴的油树脂也能够抑制NO的产生和胸膜炎，但强度较小。