解剖位置决定了树突状细胞在响应静脉注射可溶性抗原时向T细胞呈递抗原的过程。

Anatomic location defines antigen presentation by dendritic cells to T cells in response to intravenous soluble antigens.

作者信息

Chung Yeonseok, Chang Jae-Hoon, Kim Byung-Seok, Lee Jung-Mi, Kim Ho-Youn, Kang Chang-Yuil

机构信息

Laboratory of Immunology, Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul, South Korea.

出版信息

Eur J Immunol. 2007 Jun;37(6):1453-62. doi: 10.1002/eji.200636544.

DOI:10.1002/eji.200636544

PMID:17474148

Abstract

In the spleen, exogenous antigen is preferentially presented by CD8alpha+CD11b- DC to CD8 T cells and by CD8alpha-CD11b+ DC to CD4 T cells. However, it is not yet clear whether the same rule applies to other secondary lymphoid organs. To address this issue, we first classified secondary lymphoid tissues into three categories based on the expression pattern of CD8alpha and CD11b in C57BL/6 and BALB/c mice: (a) spleen, (b) mesenteric lymph node (MLN) and (c) other peripheral lymph nodes (PLN). We then analyzed the OVA-specific T cell-stimulating capacity of each DC subset after intravenous injection with soluble OVA. Our results show that, regardless of tissue origin, CD8alpha-CD11b+ DC generally present OVA to CD4 T cells, a finding that held true as well for CD8alpha+CD11b+ DC in PLN. In striking contrast, CD8alpha+CD11b- DC in spleen, CD8alpha-CD11b+ DC in MLN and CD8alpha+CD11b+ DC in PLN mainly cross-present OVA to CD8 T cells in their respective tissues. Of note, CD8alpha-CD11b+ DC in MLN and CD8alpha+CD11b+ DC in PLN present OVA to both CD4 T and CD8 T cells. Therefore, the antigen-presenting capacity of each distinct DC subset is determined by its anatomic environment in combination with its surface phenotype.

摘要

在脾脏中，外源性抗原优先由CD8α⁺CD11b⁻树突状细胞（DC）呈递给CD8 T细胞，由CD8α⁻CD11b⁺DC呈递给CD4 T细胞。然而，同样的规则是否适用于其他二级淋巴器官尚不清楚。为了解决这个问题，我们首先根据C57BL/6和BALB/c小鼠中CD8α和CD11b的表达模式，将二级淋巴组织分为三类：（a）脾脏，（b）肠系膜淋巴结（MLN）和（c）其他外周淋巴结（PLN）。然后，我们在静脉注射可溶性卵清蛋白（OVA）后，分析了每个DC亚群刺激OVA特异性T细胞的能力。我们的结果表明，无论组织来源如何，CD8α⁻CD11b⁺DC通常将OVA呈递给CD4 T细胞，PLN中的CD8α⁺CD11b⁺DC也是如此。与之形成鲜明对比的是，脾脏中的CD8α⁺CD11b⁻DC、MLN中的CD8α⁻CD11b⁺DC和PLN中的CD8α⁺CD11b⁺DC主要在各自组织中将OVA交叉呈递给CD8 T细胞。值得注意的是，MLN中的CD8α⁻CD11b⁺DC和PLN中的CD8α⁺CD11b⁺DC将OVA呈递给CD4 T细胞和CD8 T细胞。因此，每个不同DC亚群的抗原呈递能力是由其解剖学环境与其表面表型共同决定的。