靶向线粒体的活性氧荧光探针。
Mitochondrial-targeted fluorescent probes for reactive oxygen species.
机构信息
Department of Chemistry, University of California, Berkeley, CA 94720, USA.
出版信息
Curr Opin Chem Biol. 2010 Feb;14(1):50-6. doi: 10.1016/j.cbpa.2009.10.014. Epub 2009 Nov 10.
Abstract
As the primary consumers of oxygen within all aerobic organisms, mitochondria are a major source of cellular reactive oxygen species (ROS) derived from the in vivo chemistry of oxygen metabolism. Mitochondrial ROS have been traditionally implicated in aging and in a variety of pathologies, including cancer, neurodegeneration, and diabetes, but recent studies also link controlled mitochondrial ROS fluxes to cell regulation and signaling events. Progress in the development of mitochondrial-targeted fluorescent small-molecule indicators that detect specific ROS with high selectivity offers a promising approach for interrogating mitochondrial ROS production, trafficking, and downstream biological effects.
摘要
作为所有需氧生物中氧气的主要消费者，线粒体是细胞内活性氧（ROS）的主要来源，这些 ROS 来源于氧代谢的体内化学反应。线粒体 ROS 传统上与衰老和多种病理学有关，包括癌症、神经退行性疾病和糖尿病，但最近的研究也将受控的线粒体 ROS 通量与细胞调节和信号事件联系起来。开发靶向线粒体的荧光小分子指示剂的进展，这些指示剂可以高选择性地检测特定的 ROS，为研究线粒体 ROS 的产生、运输和下游生物学效应提供了一种很有前途的方法。
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本文引用的文献
1
Specific fluorogenic probes for ozone in biological and atmospheric samples.
Nat Chem. 2009 Jul;1(4):316-21. doi: 10.1038/nchem.240.
2
Targeting mitochondria with organelle-specific compounds: strategies and applications.
Chembiochem. 2009 Aug 17;10(12):1939-50. doi: 10.1002/cbic.200900185.
3
Mitochondria are the source of hydrogen peroxide for dynamic brain-cell signaling.
J Neurosci. 2009 Jul 15;29(28):9002-10. doi: 10.1523/JNEUROSCI.1706-09.2009.
4
A mitochondria-targeted S-nitrosothiol modulates respiration, nitrosates thiols, and protects against ischemia-reperfusion injury.
Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10764-9. doi: 10.1073/pnas.0903250106. Epub 2009 Jun 15.
5
Mitochondria and reactive oxygen species.
Hypertension. 2009 Jun;53(6):885-92. doi: 10.1161/HYPERTENSIONAHA.109.130054. Epub 2009 Apr 27.
6
How mitochondria produce reactive oxygen species.
Biochem J. 2009 Jan 1;417(1):1-13. doi: 10.1042/BJ20081386.
7
Expanding the functional diversity of proteins through cysteine oxidation.
Curr Opin Chem Biol. 2008 Dec;12(6):746-54. doi: 10.1016/j.cbpa.2008.07.028. Epub 2008 Sep 17.
8
Superoxide flashes in single mitochondria.
Cell. 2008 Jul 25;134(2):279-90. doi: 10.1016/j.cell.2008.06.017.
9
A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells.
J Am Chem Soc. 2008 Jul 30;130(30):9638-9. doi: 10.1021/ja802355u. Epub 2008 Jul 8.
10
The selective detection of mitochondrial superoxide by live cell imaging.
Nat Protoc. 2008;3(6):941-7. doi: 10.1038/nprot.2008.56.
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