基质辅助激光解吸电离飞行时间质谱技术用于酵母常规鉴定的性能和成本分析。
Performance and cost analysis of matrix-assisted laser desorption ionization-time of flight mass spectrometry for routine identification of yeast.
机构信息
Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, USA.
出版信息
J Clin Microbiol. 2011 Apr;49(4):1614-6. doi: 10.1128/JCM.02381-10. Epub 2011 Jan 26.
Abstract
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry was compared to phenotypic testing for yeast identification. MALDI-TOF mass spectrometry yielded 96.3% and 84.5% accurate species level identifications (spectral scores, ≥ 1.8) for 138 common and 103 archived strains of yeast. MALDI-TOF mass spectrometry is accurate, rapid (5.1 min of hands-on time/identification), and cost-effective ($0.50/sample) for yeast identification in the clinical laboratory.
摘要
基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析与表型检测用于酵母鉴定的比较。MALDI-TOF 质谱分析对 138 株常见酵母和 103 株存档酵母菌株的种水平鉴定（谱评分≥1.8）的准确率分别为 96.3%和 84.5%。MALDI-TOF 质谱分析在临床实验室中用于酵母鉴定时准确、快速（实际操作时间/鉴定 5.1 分钟）且具有成本效益（每个样本 0.50 美元）。
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