细胞形状和基质刚性都能调节细胞硬度。
Cell shape and substrate rigidity both regulate cell stiffness.
机构信息
Institute for Medicine and Engineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
出版信息
Biophys J. 2011 Mar 2;100(5):L25-7. doi: 10.1016/j.bpj.2010.12.3744.
Cells from many different tissues sense the stiffness and spatial patterning of their microenvironment to modulate their shape and cortical stiffness. It is currently unknown how substrate stiffness, cell shape, and cell stiffness modulate or interact with one another. Here, we use microcontact printing and microfabricated arrays of elastomeric posts to independently and simultaneously control cell shape and substrate stiffness. Our experiments show that cell cortical stiffness increases as a function of both substrate stiffness and spread area. For soft substrates, the influence of substrate stiffness on cell cortical stiffness is more prominent than that of cell shape, since increasing adherent area does not lead to cell stiffening. On the other hand, for cells constrained to a small area, cell shape effects are more dominant than substrate stiffness, since increasing substrate stiffness no longer affects cell stiffness. These results suggest that cell size and substrate stiffness can interact in a complex fashion to either enhance or antagonize each other's effect on cell morphology and mechanics.
来自许多不同组织的细胞感知其微环境的硬度和空间模式，以调节其形状和皮质硬度。目前尚不清楚基质硬度、细胞形状和细胞硬度如何相互调节或相互作用。在这里，我们使用微接触印刷和弹性柱微图案阵列来独立且同时控制细胞形状和基质硬度。我们的实验表明，细胞皮质硬度随基质硬度和铺展面积的增加而增加。对于软基质，基质硬度对细胞皮质硬度的影响比细胞形状更为显著，因为增加黏附面积不会导致细胞变硬。另一方面，对于被限制在小区域的细胞，细胞形状的影响比基质硬度更为显著，因为增加基质硬度不再影响细胞硬度。这些结果表明，细胞大小和基质硬度可以以复杂的方式相互作用，从而增强或拮抗彼此对细胞形态和力学的影响。
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