pH 值对阴道白色念珠菌抗真菌敏感性的影响。
Impact of pH on the antifungal susceptibility of vaginal Candida albicans.
机构信息
Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing, China.
出版信息
Int J Gynaecol Obstet. 2011 Sep;114(3):278-80. doi: 10.1016/j.ijgo.2011.03.016. Epub 2011 Jun 21.
OBJECTIVE
To investigate the antifungal susceptibility at pH 7.0 and pH 4.0 of 5 antifungal agents against Candida albicans isolated from patients with vulvovaginal candidiasis.
METHODS
Antifungal susceptibility testing at pH 7.0 and pH 4.0 was performed using the broth microdilution method (CLSI, document M27-A2).
RESULTS
The minimal inhibitory concentrations (MICs) of miconazole, clotrimazole, fluconazole, and nystatin against C. albicans at pH 4.0 were significantly higher than those at pH 7.0 (0.25 vs 0.03 μg/mL, 0.50 vs 0.03 μg/mL, 0.50 vs 0.25 μg/mL, and 32 vs 2 μg/mL, respectively; P<0.001), whereas the MIC of itraconazole at pH 4.0 was lower than that at pH 7.0 (0.030 vs 0.125 μg/mL; P<0.001). The susceptibility rate of C. albicans to itraconazole at pH 4.0 was significantly higher than at pH 7.0 (95.0% vs 51.7%; P<0.001). The susceptibility rate to itraconazole at pH 7.0 was significantly lower than the susceptibility rate to fluconazole (51.7% vs 100.0%; P<0.001), whereas the susceptibility rates to the 2 drugs were similar at pH 4.0 (95.0% and 96.7%, respectively).
CONCLUSION
Media at different pH values should be used for sensitivity tests according to the environment of C. albicans.
目的
研究 pH 值为 7.0 和 4.0 时，5 种抗真菌药物对阴道假丝酵母菌病患者分离的白色念珠菌的抗真菌药敏性。
方法
采用肉汤微量稀释法（CLSI，M27-A2 文件）进行 pH 值为 7.0 和 4.0 时的抗真菌药敏试验。
结果
pH 值为 4.0 时，咪康唑、克霉唑、氟康唑和制霉菌素对白色念珠菌的最低抑菌浓度（MIC）明显高于 pH 值为 7.0 时（0.25 对 0.03 μg/mL、0.50 对 0.03 μg/mL、0.50 对 0.25 μg/mL 和 32 对 2 μg/mL；P<0.001），而 pH 值为 4.0 时伊曲康唑的 MIC 低于 pH 值为 7.0 时（0.030 对 0.125 μg/mL；P<0.001）。pH 值为 4.0 时白色念珠菌对伊曲康唑的敏感性明显高于 pH 值为 7.0 时（95.0%对 51.7%；P<0.001）。pH 值为 7.0 时伊曲康唑的敏感性明显低于氟康唑（51.7%对 100.0%；P<0.001），而 pH 值为 4.0 时两者的敏感性相似（分别为 95.0%和 96.7%）。
结论
应根据白色念珠菌的环境，使用不同 pH 值的培养基进行药敏试验。
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