基于 BODIPY 的荧光探针可视化黑色素瘤细胞中的酪氨酸酶活性。
Visualization of tyrosinase activity in melanoma cells by a BODIPY-based fluorescent probe.
机构信息
Department of Chemistry, 126 Jukjeon-dong, Yongin-si, Gyeonggi-do, 448-701, Korea.
出版信息
Chem Commun (Camb). 2011 Dec 21;47(47):12640-2. doi: 10.1039/c1cc15061h. Epub 2011 Oct 26.
PMID:22031105
Abstract
We have presented a fluorescent probe 1 that exhibits a fluorescence turn-on signal upon reaction with tyrosinase, and we show that it is readily employed for the assessment of tyrosinase activity and tyrosinase inhibitor activity in buffered aqueous solution, and further utilized for the visualization of endogenous tyrosinase activity in living melanoma cells.
摘要
我们提出了一种荧光探针 1，它在与酪氨酸酶反应时表现出荧光开启信号，我们表明它可用于在缓冲水溶液中评估酪氨酸酶活性和酪氨酸酶抑制剂活性，并进一步用于可视化活黑素瘤细胞中的内源性酪氨酸酶活性。
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