评估局部抗甲氧西林金黄色葡萄球菌生物膜对浅表性小鼠伤口抗菌疗效的模型。
A model for evaluating topical antimicrobial efficacy against methicillin-resistant Staphylococcus aureus biofilms in superficial murine wounds.
机构信息
Research and Development, Healthpoint Biotherapeutics, Fort Worth, Texas, USA.
出版信息
Antimicrob Agents Chemother. 2012 Aug;56(8):4508-10. doi: 10.1128/AAC.00467-12. Epub 2012 May 29.
Abstract
A wound biofilm model was created by adapting a superficial infection model. Partial-thickness murine wounds were inoculated with methicillin-resistant Staphylococcus aureus (MRSA). Dense biofilm communities developed at the wound surface after 24 h as demonstrated by microscopy and quantitative microbiology. Common topical antimicrobial agents had reduced efficacy when treatment was initiated 24 h after inoculation compared to 4 h after inoculation. This model provides a rapid in vivo test for new agents to treat wound biofilm infections.
摘要
一种创伤生物膜模型是通过改编一种浅表感染模型而创建的。部分厚度的小鼠创伤用耐甲氧西林金黄色葡萄球菌（MRSA）接种。在 24 小时后，显微镜和定量微生物学显示，在创伤表面形成了密集的生物膜群落。与接种后 4 小时相比，当治疗在接种后 24 小时开始时，常见的局部抗菌剂的疗效降低。该模型为治疗创伤生物膜感染的新药物提供了一种快速的体内测试方法。
相似文献
1
A model for evaluating topical antimicrobial efficacy against methicillin-resistant Staphylococcus aureus biofilms in superficial murine wounds.
Antimicrob Agents Chemother. 2012 Aug;56(8):4508-10. doi: 10.1128/AAC.00467-12. Epub 2012 May 29.
2
Cadexomer iodine provides superior efficacy against bacterial wound biofilms in vitro and in vivo.
Wound Repair Regen. 2017 Jan;25(1):13-24. doi: 10.1111/wrr.12497. Epub 2016 Dec 5.
3
Dealing with biofilms of Pseudomonas aeruginosa and Staphylococcus aureus: In vitro evaluation of a novel aerosol formulation of silver sulfadiazine.
Burns. 2020 Feb;46(1):128-135. doi: 10.1016/j.burns.2019.07.027. Epub 2019 Aug 14.
4
In vitro susceptibility testing of topical antimicrobial agents used in pediatric burn patients: comparison of two methods.
J Burn Care Rehabil. 1997 Sep-Oct;18(5):406-10. doi: 10.1097/00004630-199709000-00006.
5
"Smart" polymer enhances the efficacy of topical antimicrobial agents.
Burns. 2019 Sep;45(6):1418-1429. doi: 10.1016/j.burns.2019.04.013. Epub 2019 Jun 21.
6
Octenidine dihydrochloride treatment of a meticillin-resistant biofilm-infected mouse wound.
J Wound Care. 2021 Feb 2;30(2):106-114. doi: 10.12968/jowc.2021.30.2.106.
7
[Investigation of antibacterial activity of topical antimicrobials against methicillin-resistant Staphylococcus aureus].
Zhonghua Shao Shang Za Zhi. 2014 Feb;30(1):21-4.
8
Antimicrobial effects of a multimodal wound matrix against methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa in an in vitro and an in vivo porcine wound model.
Int Wound J. 2024 Oct;21(10):e70059. doi: 10.1111/iwj.70059.
9
Inflammatory and antimicrobial responses to methicillin-resistant Staphylococcus aureus in an in vitro wound infection model.
PLoS One. 2013 Dec 10;8(12):e82800. doi: 10.1371/journal.pone.0082800. eCollection 2013.
10
Development of Chitosan-Based Hydrogel Containing Antibiofilm Agents for the Treatment of Staphylococcus aureus-Infected Burn Wound in Mice.
AAPS PharmSciTech. 2020 Jan 2;21(2):43. doi: 10.1208/s12249-019-1537-2.
引用本文的文献
1
Biofilm-Associated Infections in Chronic Wounds and Their Management.
Adv Exp Med Biol. 2023;1370:55-75. doi: 10.1007/5584_2022_738.
2
Enzymatically-crosslinked gelatin hydrogels containing paenipeptin and clarithromycin against carbapenem-resistant pathogen in murine skin wound infection.
BMC Microbiol. 2021 Nov 24;21(1):326. doi: 10.1186/s12866-021-02383-z.
3
Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both.
Front Med (Lausanne). 2021 May 25;8:673408. doi: 10.3389/fmed.2021.673408. eCollection 2021.
4
Evaluation of Bacterial RNA Polymerase Inhibitors in a -Based Wound Infection Model in SKH1 Mice.
ACS Infect Dis. 2020 Oct 9;6(10):2573-2581. doi: 10.1021/acsinfecdis.0c00034. Epub 2020 Sep 21.
5
Enantiomeric glycosylated cationic block co-beta-peptides eradicate Staphylococcus aureus biofilms and antibiotic-tolerant persisters.
Nat Commun. 2019 Oct 21;10(1):4792. doi: 10.1038/s41467-019-12702-8.
6
A Dual-Function Antibiotic-Transporter Conjugate Exhibits Superior Activity in Sterilizing MRSA Biofilms and Killing Persister Cells.
J Am Chem Soc. 2018 Nov 28;140(47):16140-16151. doi: 10.1021/jacs.8b08711. Epub 2018 Nov 14.
7
MRSA decolonization failure-are biofilms the missing link?
Antimicrob Resist Infect Control. 2017 Mar 28;6:32. doi: 10.1186/s13756-017-0192-1. eCollection 2017.
8
Injections through skin colonized with Staphylococcus aureus biofilm introduce contamination despite standard antimicrobial preparation procedures.
Sci Rep. 2017 Mar 23;7:45070. doi: 10.1038/srep45070.
9
Newly Developed Topical Cefotaxime Sodium Hydrogels: Antibacterial Activity and In Vivo Evaluation.
Biomed Res Int. 2016;2016:6525163. doi: 10.1155/2016/6525163. Epub 2016 May 24.
10
An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue.
BMC Res Notes. 2016 Apr 12;9:216. doi: 10.1186/s13104-016-1902-0.
本文引用的文献
1
Treatment of Pseudomonas aeruginosa biofilm-infected wounds with clinical wound care strategies: a quantitative study using an in vivo rabbit ear model.
Plast Reconstr Surg. 2012 Feb;129(2):262e-274e. doi: 10.1097/PRS.0b013e31823aeb3b.
2
An in vitro model for the growth and analysis of chronic wound MRSA biofilms.
J Appl Microbiol. 2011 Nov;111(5):1275-82. doi: 10.1111/j.1365-2672.2011.05138.x. Epub 2011 Sep 19.
3
Development of a novel, highly quantitative in vivo model for the study of biofilm-impaired cutaneous wound healing.
Wound Repair Regen. 2011 May-Jun;19(3):400-10. doi: 10.1111/j.1524-475X.2011.00690.x. Epub 2011 Apr 21.
4
Antimicrobial tolerance and the significance of persister cells in recalcitrant chronic wound biofilms.
Wound Repair Regen. 2011 Jan-Feb;19(1):1-9. doi: 10.1111/j.1524-475X.2010.00651.x.
5
Delayed wound healing in diabetic (db/db) mice with Pseudomonas aeruginosa biofilm challenge: a model for the study of chronic wounds.
Wound Repair Regen. 2010 Sep-Oct;18(5):467-77. doi: 10.1111/j.1524-475X.2010.00608.x. Epub 2010 Aug 19.
6
Staphylococcal biofilms impair wound healing by delaying reepithelialization in a murine cutaneous wound model.
Wound Repair Regen. 2009 May-Jun;17(3):354-9. doi: 10.1111/j.1524-475X.2009.00489.x.
7
Evaluation of analogs of reutericyclin as prospective candidates for treatment of staphylococcal skin infections.
Antimicrob Agents Chemother. 2009 Sep;53(9):4028-31. doi: 10.1128/AAC.00457-09. Epub 2009 Jul 6.
8
Distribution, organization, and ecology of bacteria in chronic wounds.
J Clin Microbiol. 2008 Aug;46(8):2717-22. doi: 10.1128/JCM.00501-08. Epub 2008 May 28.
9
Biofilms in chronic wounds.
Wound Repair Regen. 2008 Jan-Feb;16(1):37-44. doi: 10.1111/j.1524-475X.2007.00321.x. Epub 2007 Dec 13.
10
Neutropenia induced in outbred mice by a simplified low-dose cyclophosphamide regimen: characterization and applicability to diverse experimental models of infectious diseases.
BMC Infect Dis. 2006 Mar 17;6:55. doi: 10.1186/1471-2334-6-55.
Zotero 插件