采用 FilmArray 系统的多重聚合酶链反应快速鉴定血培养阳性标本中的病原体。
Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system.
机构信息
Department of Pediatrics, University of Utah, Salt Lake City, UT, USA.
出版信息
Diagn Microbiol Infect Dis. 2012 Dec;74(4):349-55. doi: 10.1016/j.diagmicrobio.2012.08.013. Epub 2012 Sep 19.
Abstract
Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.
摘要
败血症是主要死因。直接从血培养中快速准确地鉴定病原体和抗菌药物耐药性可以改善患者的预后。FilmArray®（FA；美国犹他州盐湖城的 Idaho Technology）血培养（BC）试剂盒在 1 小时内可以从阳性血培养物中鉴定出 >25 种病原体和 4 种抗生素耐药基因。我们将该试剂盒的开发版本与常规培养和药敏试验对 102 份来自成人和儿童菌血症患者的存档血培养物进行了比较。在通过培养鉴定的 109 种病原体中，95%通过 FA 鉴定。在 111 份前瞻性采集的血培养物中，FA 鉴定出了 84 种（91%）涵盖在试剂盒中的病原体。在 25 株金黄色葡萄球菌和 21 株肠球菌中，FA 鉴定出了所有经培养证实的耐甲氧西林金黄色葡萄球菌和耐万古霉素肠球菌。FA BC 试剂盒是一种快速鉴定血培养物中病原体和耐药基因的准确方法。
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本文引用的文献
1
Deaths: preliminary data for 2009.
Natl Vital Stat Rep. 2011 Mar;59(4):1-51.
2
FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection.
PLoS One. 2011;6(10):e26047. doi: 10.1371/journal.pone.0026047. Epub 2011 Oct 19.
3
An efficient strategy for broad-range detection of low abundance bacteria without DNA decontamination of PCR reagents.
PLoS One. 2011;6(5):e20303. doi: 10.1371/journal.pone.0020303. Epub 2011 May 26.
4
Loss of vancomycin resistance not completely dependent on the Tn1546 element in Enterococcus faecium isolates.
Diagn Microbiol Infect Dis. 2011 Jan;69(1):105-10. doi: 10.1016/j.diagmicrobio.2010.08.030.
5
Database resources of the National Center for Biotechnology Information.
Nucleic Acids Res. 2011 Jan;39(Database issue):D38-51. doi: 10.1093/nar/gkq1172. Epub 2010 Nov 21.
6
GenBank.
Nucleic Acids Res. 2011 Jan;39(Database issue):D32-7. doi: 10.1093/nar/gkq1079. Epub 2010 Nov 10.
7
An antimicrobial stewardship program's impact with rapid polymerase chain reaction methicillin-resistant Staphylococcus aureus/S. aureus blood culture test in patients with S. aureus bacteremia.
Clin Infect Dis. 2010 Nov 1;51(9):1074-80. doi: 10.1086/656623.
8
Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.
Transfus Med Hemother. 2010 Feb;37(1):21-28. doi: 10.1159/000265571. Epub 2010 Jan 7.
9
Multicenter evaluation of a new shortened peptide nucleic acid fluorescence in situ hybridization procedure for species identification of select Gram-negative bacilli from blood cultures.
J Clin Microbiol. 2010 Jun;48(6):2268-70. doi: 10.1128/JCM.00166-10. Epub 2010 Mar 31.
10
The era of molecular and other non-culture-based methods in diagnosis of sepsis.
Clin Microbiol Rev. 2010 Jan;23(1):235-51. doi: 10.1128/CMR.00043-09.
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