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[对18 - 45岁年龄组女性阴道分泌物标本进行阴道毛滴虫及其他微生物的显微镜检查]

[Microscopic examination of vaginal discharge specimens for Trichomonas vaginalis and other micro-organisms in 18-45 age group women].

作者信息

Keşli Recep, Pektaş Bayram, Ozdemir Mehmet, Günenc Oğuzhan, Coşkun Erkan, Baykan Mahmut, Baysal Bülent

机构信息

Konya Eğitim ve Araştırma Hastanesi, Tıbbi Mikrobiyoloji Laboratuvarı, Konya, Turkiye.

出版信息

Turkiye Parazitol Derg. 2012;36(3):182-4. doi: 10.5152/tpd.2012.43.

Abstract

OBJECTIVE

Trichomonas vaginalis is a protozoon that causes trichomoniasis which is characterised by a foamy yellowish odorous discharge and superficial defects and necrotic ulcers in vaginal mucosa. Trichomoniasis is transmitted from human to human by sexual contact and can be seen in almost every part of the world. The aim of this study was to determine the incidence of Trichomonas vaginalis in 18-45 years age group women with vaginal discharge complaints who applied to the Gynaecology Outpatient Clinic of Konya Social Insurance Instution Hospital during September 1-December 15 2003.

METHODS

Samples were taken from posterior fornix of the vagina with the aid of a speculum and sterile cotton swabs. All the samples were examined by wet mount preparations, Gram and Giemsa staining method under the light microscope.

RESULTS

Of seventy samples 6 (9%) were positive for Trichomonas vaginalis, 9 (13%) for Gardnerella vaginalis, one for Mobiluncus spp. and 11 (16%) for Candida spp.

CONCLUSION

It is possible to say that, in spite of a definite diagnosis of trichomoniasis made by cultivation method, examining the vaginal smear by direct microscope also has an important role in the diagnosis of infection. Direct microscopic examination will help in deciding whether to begin the treatment of trichomoniasis.

摘要

目的

阴道毛滴虫是一种原生动物,可引起滴虫病,其特征为阴道排出泡沫状、黄色且有异味的分泌物,以及阴道黏膜出现浅表缺损和坏死性溃疡。滴虫病通过性接触在人与人之间传播,在世界几乎每个地区都可见到。本研究的目的是确定2003年9月1日至12月15日期间前往科尼亚社会保险机构医院妇科门诊就诊、有阴道分泌物异常主诉的18至45岁女性中阴道毛滴虫的感染率。

方法

借助窥器和无菌棉拭子从阴道后穹窿取样。所有样本均在光学显微镜下通过湿片制备、革兰氏染色和吉姆萨染色法进行检查。

结果

在70份样本中,6份(9%)阴道毛滴虫检测呈阳性,9份(13%)阴道加德纳菌检测呈阳性,1份动弯杆菌属检测呈阳性,11份(16%)念珠菌属检测呈阳性。

结论

尽管培养法对滴虫病有明确诊断,但直接显微镜检查阴道涂片在感染诊断中也具有重要作用。直接显微镜检查有助于决定是否开始滴虫病的治疗。

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