基于双功能金纳米粒子的比色传感器：分析物识别和过氧化物酶样活性。

Colorimetric sensor based on dual-functional gold nanoparticles: analyte-recognition and peroxidase-like activity.

机构信息

Department of Pharmaceutical Analysis, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China; Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou 350004, China.

出版信息

Food Chem. 2014 Mar 15;147:257-61. doi: 10.1016/j.foodchem.2013.09.151. Epub 2013 Oct 9.

DOI:10.1016/j.foodchem.2013.09.151

PMID:24206715

Abstract

A novel colorimetric sensor based on the interaction ability with specific analytes and peroxidase-like activity of gold nanoparticles was established in this work. Combining the high-affinity binding between bare gold nanoparticles and melamine with signal amplification procedure based on the catalytic activity of gold nanoparticles for oxidation of TMB, melamine with the concentration as low as 0.02 mg/L can be easily distinguished by naked-eye observation. Such system can be adapted through carefully-controlled surface modifications of gold nanoparticles for determination of other targets.

摘要

在这项工作中，建立了一种基于与特定分析物相互作用能力和纳米金的过氧化物酶样活性的新型比色传感器。结合裸金纳米粒子与三聚氰胺之间的高亲和力结合以及基于金纳米粒子对 TMB 氧化的催化活性的信号放大过程，即使三聚氰胺的浓度低至 0.02mg/L，也可以通过肉眼观察轻松区分。通过仔细控制金纳米粒子的表面修饰，可以将该系统适用于测定其他靶标。