利用 CRISPR-Cas9 系统对人类疟原虫（Plasmodium falciparum）进行基因组编辑。
Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system.
机构信息
1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France. [3].
1] Biology of Host-Parasite Interactions Unit, Institut Pasteur, Paris, France. [2] CNRS URA 2581, Institut Pasteur, Paris, France.
出版信息
Nat Biotechnol. 2014 Aug;32(8):819-21. doi: 10.1038/nbt.2925. Epub 2014 Jun 1.
Genome manipulation in the malaria parasite Plasmodium falciparum remains largely intractable and improved genomic tools are needed to further understand pathogenesis and drug resistance. We demonstrated the CRISPR-Cas9 system for use in P. falciparum by disrupting chromosomal loci and generating marker-free, single-nucleotide substitutions with high efficiency. Additionally, an artemisinin-resistant strain was generated by introducing a previously implicated polymorphism, thus illustrating the value of efficient genome editing in malaria research.
疟原虫属疟原虫中的基因组操作在很大程度上仍然难以实现，需要改进基因组工具来进一步了解发病机制和耐药性。我们通过破坏染色体基因座并高效生成无标记、单核苷酸替换，证明了 CRISPR-Cas9 系统在疟原虫属疟原虫中的应用。此外，还通过引入先前涉及的多态性产生了对青蒿素的抗性株，从而说明了高效基因组编辑在疟疾研究中的价值。
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