[宫颈癌高危女性中抗人乳头瘤病毒16型和18型血清抗体的分布情况]

Fei Mandong, Li Jiayuan, Du Jingchang, You Jia, Zhang Shaokai, He Wei, Kang Leni, Zhao Fanghui, Qiao Youlin, Si Yuzhi, Fan Xiaoping, Chen Wen

Cancer Institute and Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China; Department of Epidemiology, West China School of Public Health, Sichuan University.

Department of Epidemiology, West China School of Public Health, Sichuan University.

Zhonghua Liu Xing Bing Xue Za Zhi. 2014 May;35(5):514-8.

OBJECTIVE

To explore the distribution of serum antibodies against human papillomavirus (HPV) 16/18 among women at high-risk for cervical cancer.

METHODS

All women when tested positive for anyone of the cervical cancer screening programs, from Xinmi county of Henan province in 2011, were recruited as the subjects of this study. Cervical exfoliated cells were collected, using cervical brush for HPV DNA testing, and 10 ml venous blood was drawn for HPV-16, 18 serum antibodies testing, by enzyme-linked immunosorbent assay.

RESULTS

Among the 952 women under study, 230 cases (24.2%)showed HPV DNA positive, with positivity rates of HPV16 and 18 L1 virus-like particle (VLP)antibodies as 23.2% and 6.5%, respectively. The overall positivity rate of any type of HPV16, 18 VLP antibodies was 26.8% . Geometric means of HPV16, 18 VLP antibody titers were 79.1 (Yangshengtang Unit,YU/ml) and 125.0(YU/ml). Positivity rate of HPV16 antibody was significantly associated with age, viral load of HPV DNA, and cervical lesion severity (P < 0.05). Seropositivity of HPV18 was also increasing with the increase of viral load (P < 0.01) with different cervical lesion significantly showing different titer of HPV18 antibody (P < 0.01). Based on the results of HPV DNA detection among the two years of study, women with HPV persistent infection showed significant higher positive rate of HPV16/18 antibodies than women who did not have HPV infection or emerging infection (P < 0.001). When comparing to those women without HPV infection, the ones with transient infection showed higher seropositivity rates on both HPV16 antibodies and titer of HPV16 antibody (P < 0.001).

CONCLUSION

Seroprevalence rates on HPV16 and 18 among the unvaccinated high-risk women in Henan were high. Prevalence of both HPV16 and 18 antibodies were correlated with age, viral load, cervical lesion and history of infection.Women with high viral load, high grade cervical lesion or history of infection would more likely to be seropositive.

目的

探讨宫颈癌高危女性中人乳头瘤病毒（HPV）16/18血清抗体的分布情况。

方法

选取2011年河南省新密市宫颈癌筛查项目中任何一项检测呈阳性的所有女性作为本研究对象。采集宫颈脱落细胞，用宫颈刷进行HPV DNA检测，并抽取10 ml静脉血，采用酶联免疫吸附试验检测HPV-16、18血清抗体。

结果

在952例研究对象中，230例（24.2%）HPV DNA呈阳性，HPV16和18 L1病毒样颗粒（VLP）抗体阳性率分别为23.2%和6.5%。任何一种HPV16、18 VLP抗体的总体阳性率为26.8%。HPV16、18 VLP抗体滴度的几何平均值分别为79.1（养生堂单位，YU/ml）和125.0（YU/ml）。HPV16抗体阳性率与年龄、HPV DNA病毒载量及宫颈病变严重程度显著相关（P<0.05）。HPV18血清阳性率也随病毒载量增加而升高（P<0.01），不同宫颈病变HPV18抗体滴度差异有统计学意义（P<0.01）。根据两年研究中的HPV DNA检测结果，HPV持续感染的女性HPV16/18抗体阳性率显著高于未感染或新发感染的女性（P<0.001）。与未感染HPV的女性相比，短暂感染的女性HPV16抗体阳性率及HPV16抗体滴度均较高（P<0.001）。