一种基于罗丹明B的新型“开-关”型化学传感器，对Fe(3+)具有高选择性和高灵敏度及其在活细胞中的成像
A new Rhodamine B-based 'on-off' chemical sensor with high selectivity and sensitivity toward Fe(3+) and its imaging in living cells.
作者信息
Bao Xiaofeng, Shi Jiaxin, Nie Xuemei, Zhou Baojing, Wang Xinlong, Zhang Luyong, Liao Hong, Pang Tao
机构信息
Department of Biochemical Engineering, Nanjing University of Science & Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094, PR China.
School of Chemical Engineering, Nanjing University of Science & Technology, Nanjing 210094, PR China.
出版信息
Bioorg Med Chem. 2014 Sep 1;22(17):4826-35. doi: 10.1016/j.bmc.2014.06.054. Epub 2014 Jul 8.
A new fluorescent chemosensor based on a Rhodamine B and pyrrole conjugate (RBPY) has been designed and synthesized. UV-vis absorption and fluorescence spectroscopic studies show that RBPY exhibits a high selectivity and sensitivity toward Fe(3+) among many other metal cations in a MeOH/H2O solution (3:2, v/v, pH 7.10, HEPES buffer, 0.1mM) by forming a 1:1 complex with Fe(3+). Furthermore, results reveal that the formation of the RBPY-Fe(3+) complex is fully reversible in the presence of sulfide anions and could also be used as an efficient sensor for S(2-). Importantly, fluorescence microscopy experiments further demonstrated that RBPY can be utilized as a fluorescent probe for the detection of Fe(3+) in human liver (L-02) cells.
一种基于罗丹明B和吡咯共轭物（RBPY）的新型荧光化学传感器已被设计并合成。紫外-可见吸收光谱和荧光光谱研究表明，在甲醇/水（3:2，v/v，pH 7.10，HEPES缓冲液，0.1 mM）溶液中，RBPY在许多其他金属阳离子中对Fe(3+)表现出高选择性和灵敏度，通过与Fe(3+)形成1:1络合物实现。此外，结果表明，在硫化物阴离子存在下，RBPY-Fe(3+)络合物的形成是完全可逆的，并且还可以用作S(2-)的有效传感器。重要的是，荧光显微镜实验进一步证明，RBPY可作为荧光探针用于检测人肝细胞（L-02）中的Fe(3+)。
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