一种萘-吡唑共轭物：适用于水溶液中作为生物标志物的铝（III）离子选择性蓝移化学传感器。

A napthelene-pyrazol conjugate: Al(III) ion-selective blue shifting chemosensor applicable as biomarker in aqueous solution.

作者信息

Mukherjee Manjira, Pal Siddhartha, Lohar Somenath, Sen Buddhadeb, Sen Supriti, Banerjee Samya, Banerjee Snehasis, Chattopadhyay Pabitra

机构信息

Department of Chemistry, Burdwan University, Golapbag, Burdwan-713104, West Bengal, India.

出版信息

Analyst. 2014 Oct 7;139(19):4828-35. doi: 10.1039/c4an01039f.

DOI:10.1039/c4an01039f

PMID:25075382

Abstract

A newly synthesized and crystalographically characterized napthelene–pyrazol conjugate, 1-[(5-phenyl-1H-pyrazole-3-ylimino)-methyl]-naphthalen-2-ol (HL) behaves as an Al(III) ion-selective chemosensor through internal charge transfer (ICT)-chelation-enhanced fluorescence (CHEF) processes in 100 mM HEPES buffer (water–DMSO 5:1, v/v) at biological pH with almost no interference of other competitive ions. This mechanism is readily studied from electronic, fluorimetric and (1)H NMR titration. The probe (HL) behaved as a highly selective fluorescent sensor for Al(III) ions as low as 31.78 nM within a very short response time (15–20 s). The sensor (HL), which has no cytotoxicity, is also efficient in detecting the distribution of Al(III) ions in HeLa cells via image development under fluorescence microscope.

摘要

一种新合成并经晶体学表征的萘 - 吡唑共轭物，1 - [(5 - 苯基 - 1H - 吡唑 - 3 - 基亚氨基)-甲基]-萘 - 2 - 醇（HL），在100 mM HEPES缓冲液（水 - 二甲基亚砜5:1，v/v）中，于生物pH值下，通过内电荷转移（ICT）-螯合增强荧光（CHEF）过程，作为一种铝（III）离子选择性化学传感器，几乎不受其他竞争离子的干扰。这种机制可通过电子、荧光和¹H NMR滴定轻松研究。该探针（HL）在非常短的响应时间（15 - 20秒）内，对低至31.78 nM的铝（III）离子表现为高选择性荧光传感器。该无细胞毒性的传感器（HL），通过荧光显微镜下的成像，在检测HeLa细胞中铝（III）离子分布方面也很有效。