糖生植物拟南芥和盐生植物盐芥中醛脱氢酶（ALDH）基因超家族的比较研究。
Comparative study of the aldehyde dehydrogenase (ALDH) gene superfamily in the glycophyte Arabidopsis thaliana and Eutrema halophytes.
作者信息
Hou Quancan, Bartels Dorothea
机构信息
Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Kirschallee 1, D-53315 Bonn, Germany.
Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, Kirschallee 1, D-53315 Bonn, Germany
出版信息
Ann Bot. 2015 Feb;115(3):465-79. doi: 10.1093/aob/mcu152. Epub 2014 Aug 1.
BACKGROUND AND AIMS
Stresses such as drought or salinity induce the generation of reactive oxygen species, which subsequently cause excessive accumulation of aldehydes in plant cells. Aldehyde dehydrogenases (ALDHs) are considered as 'aldehyde scavengers' to eliminate toxic aldehydes caused by oxidative stress. The completion of the genome sequencing projects of the halophytes Eutrema parvulum and E. salsugineum has paved the way to explore the relationships and the roles of ALDH genes in the glycophyte Arabidopsis thaliana and halophyte model plants.
METHODS
Protein sequences of all plant ALDH families were used as queries to search E. parvulum and E. salsugineum genome databases. Evolutionary analyses compared the phylogenetic relationships of ALDHs from A. thaliana and Eutrema. Expression patterns of several stress-associated ALDH genes were investigated under different salt conditions using reverse transcription-PCR. Putative cis-elements in the promoters of ALDH10A8 from A. thaliana and E. salsugineum were compared in silico.
KEY RESULTS
Sixteen and 17 members of ten ALDH families were identified from E. parvulum and E. salsugineum genomes, respectively. Phylogenetic analysis of ALDH protein sequences indicated that Eutrema ALDHs are closely related to those of Arabidopsis, and members within these species possess nearly identical exon-intron structures. Gene expression analysis under different salt conditions showed that most of the ALDH genes have similar expression profiles in Arabidopsis and E. salsugineum, except for ALDH7B4 and ALDH10A8. In silico analysis of promoter regions of ALDH10A8 revealed different distributions of cis-elements in E. salsugineum and Arabidopsis.
CONCLUSIONS
Genomic organization, copy number, sub-cellular localization and expression profiles of ALDH genes are conserved in Arabidopsis, E. parvulum and E. salsugineum. The different expression patterns of ALDH7B4 and ALDH10A8 in Arabidopsis and E. salsugineum suggest that E. salsugineum uses modified regulatory pathways, which may contribute to salinity tolerance.
背景与目的
干旱或盐度等胁迫会诱导活性氧的产生，进而导致植物细胞中醛类物质过度积累。醛脱氢酶（ALDHs）被视为“醛清除剂”，可消除氧化应激产生的有毒醛类。盐生植物小盐芥和盐芥基因组测序项目的完成，为探索ALDH基因在甜土植物拟南芥和盐生植物模式植物中的关系及作用铺平了道路。
方法
以所有植物ALDH家族的蛋白质序列为查询序列，搜索小盐芥和盐芥的基因组数据库。进化分析比较了拟南芥和盐芥中ALDHs的系统发育关系。使用逆转录PCR研究了几种与胁迫相关的ALDH基因在不同盐条件下的表达模式。对拟南芥和盐芥中ALDH10A8启动子中的假定顺式作用元件进行了电子比较。
关键结果
分别从小盐芥和盐芥基因组中鉴定出10个ALDH家族的16个和17个成员。ALDH蛋白质序列的系统发育分析表明，盐芥ALDHs与拟南芥的密切相关，且这些物种中的成员具有几乎相同的外显子-内含子结构。不同盐条件下的基因表达分析表明，除ALDH7B4和ALDH10A8外，大多数ALDH基因在拟南芥和盐芥中具有相似的表达谱。对ALDH10A8启动子区域的电子分析揭示了盐芥和拟南芥中顺式作用元件的不同分布。
结论
ALDH基因的基因组组织、拷贝数、亚细胞定位和表达谱在拟南芥、小盐芥和盐芥中是保守的。ALDH7B4和ALDH10A8在拟南芥和盐芥中的不同表达模式表明，盐芥使用了经过修饰的调控途径，这可能有助于其耐盐性。
Zotero 插件