黏蛋白大分子与钙-海藻酸盐凝胶在分子水平上的黏附作用。

Work of adhesion between mucin macromolecule and calcium-alginate gels on molecular level.

机构信息

Institute of Physics, Faculty for Natural Sciences and Mathematics, Skopje, Macedonia.

出版信息

Carbohydr Polym. 2015 Jun 5;123:146-9. doi: 10.1016/j.carbpol.2015.01.033. Epub 2015 Jan 29.

DOI:10.1016/j.carbpol.2015.01.033

Abstract

The bioadhesion of biopolymers to mucus layers is of great interest for the development of drug delivery systems. Herein we use AFM force measurements to evaluate the interaction on molecular level between a mucin macromolecule attached to an AFM tip and a calcium-alginate gel layer. The total work of adhesion is measured from the AFM force curves depending on different parameters: time of contact, G/M ratio of the alginate, and crosslink ratio of the gel. The total work of adhesion is found to be in the range of 1×10(-19) to 6×10(-18)J. The results show that the work of adhesion increases with the time of contact but it is independent from the molecular mass of the alginate, the G/M ratio of the alginate and crosslink ratio of the gel.

摘要

生物聚合物与黏液层的生物附着对于药物输送系统的发展非常重要。本文使用原子力显微镜（AFM）力测量来评估附着在 AFM 尖端上的黏蛋白大分子与钙-海藻酸钠凝胶层之间在分子水平上的相互作用。根据不同的参数，从 AFM 力曲线测量粘附的总功，这些参数包括接触时间、海藻酸钠的 G/M 比以及凝胶的交联比。粘附的总功在 1×10(-19) 到 6×10(-18)J 范围内。结果表明，粘附功随接触时间的增加而增加，但与海藻酸钠的分子量、海藻酸钠的 G/M 比和凝胶的交联比无关。