蜡样芽孢杆菌群噬菌体JBP901的全基因组序列及系统发育位置
Complete genome sequence and phylogenetic position of the Bacillus cereus group phage JBP901.
作者信息
Asare Paul Tetteh, Ryu Sangryeol, Kim Kwang-Pyo
机构信息
Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Korea.
出版信息
Arch Virol. 2015 Sep;160(9):2381-4. doi: 10.1007/s00705-015-2485-0. Epub 2015 Jul 4.
Bacteriophage JBP901, isolated from fermented food, is specific for Bacillus cereus group species and exhibits a broad host spectrum among a large number of B. cereus isolates. Genome sequence analysis revealed a linear 159,492-bp genome with overall G+C content of 39.7 mol%, and 201 ORFs. The presence of a putative methylase, the large number of tRNAs, and the large number of nucleotide-metabolism- and replication-related genes in JBP901 reflects its broad lytic capacity. Most of the ORFs showed a high degree of similarity to Bcp1, Bc431v3 and BCP78, and various comparative genomics analyses also consistently clustered JBP901 with orphan (unclassified) Bacillus phages in the subfamily Spounavirinae of the family Myoviridae, supporting the presence of a distinguishable group in the subfamily.
从发酵食品中分离出的噬菌体JBP901对蜡样芽孢杆菌群物种具有特异性，并且在大量蜡样芽孢杆菌分离株中表现出广泛的宿主谱。基因组序列分析显示其基因组呈线性，长度为159,492 bp，总体G+C含量为39.7 mol%，共有201个开放阅读框（ORF）。JBP901中存在一个推定的甲基化酶、大量的转运RNA（tRNA）以及大量与核苷酸代谢和复制相关的基因，这反映了其广泛的裂解能力。大多数开放阅读框与Bcp1、Bc431v3和BCP78具有高度相似性，各种比较基因组学分析也一致将JBP901与肌尾噬菌体科斯波纳病毒亚科中的孤儿（未分类）芽孢杆菌噬菌体归为一类，支持了该亚科中存在一个可区分的类群。
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