黑芥子（十字花科）种子80%甲醇提取物对小鼠伯氏疟原虫感染的抗疟活性。
Antimalarial activity of 80 % methanolic extract of Brassica nigra (L.) Koch. (Brassicaceae) seeds against Plasmodium berghei infection in mice.
作者信息
Muluye Abrham Belachew, Melese Eshetie, Adinew Getnet Mequanint
机构信息
Department of Pharmacy, Wollega University, Nekemte, Ethiopia.
Department of Pharmacology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
出版信息
BMC Complement Altern Med. 2015 Oct 15;15:367. doi: 10.1186/s12906-015-0893-z.
BACKGROUND
Resistances to currently available drugs and insecticides, significant drug toxicities and costs and lack of vaccines currently complicated the treatment of malaria. A continued search for safe, effective and affordable plant-based antimalarial agents thus becomes crucial and vital in the face of these difficulties. The aim of the study was to evaluate the antimalarial activity of 80 % methanolic extract of the seeds of Brassica nigra against Plasmodium berghei infection in mice.
METHOD
Chloroquine sensitive Plasmodium berghei (ANKA strain) was used to test the antimalarial activity of the extract. In suppressive and prophylactic models, Swiss albino male mice were randomly grouped into five groups of five mice each. Group I mice were treated with the vehicle, group II, III and IV were treated with 100, 200, and 400 mg/kg of the extract, respectively and the last group (V) mice were treated with chloroquine (10 mg/kg). The level of parasitemia, survival time and variation in weight of mice were used to determine the antimalarial activity of the extract.
RESULTS
Chemosuppressive activities produced by the extract of the seeds of Brassica nigra were 21.88, 50.00 (P < 0.01) and 53.13 % (P < 0.01), while the chemoprophylactic activities were 17.42, 21.21 and 53.79 % (P < 0.05) at 100, 200 and 400 mg/kg of the extract, respectively as compared to the negative control. Mice treated with 200 and 400 mg/kg extract were significantly (P < 0.05) lived longer and gained weight as compared to negative control in 4-day suppressive test.
CONCLUSION
From this study, it can be concluded that the seed extract of Brassica nigra showed good chemosuppressive and moderate chemoprophylactic activities and the plant may contain biologically active principles which are relevant in the treatment and prophylaxis of malaria, thus supporting further studies of the plant for its active components.
背景
对现有药物和杀虫剂的耐药性、显著的药物毒性和成本以及目前缺乏疫苗，使得疟疾治疗变得复杂。面对这些困难，持续寻找安全、有效且经济实惠的植物性抗疟药物变得至关重要。本研究的目的是评估黑芥子种子80%甲醇提取物对小鼠伯氏疟原虫感染的抗疟活性。
方法
使用氯喹敏感的伯氏疟原虫（ANKA株）来测试提取物的抗疟活性。在抑制和预防模型中，将瑞士白化雄性小鼠随机分为五组，每组五只。第一组小鼠用赋形剂处理，第二、三、四组小鼠分别用100、200和400mg/kg的提取物处理，最后一组（第五组）小鼠用氯喹（10mg/kg）处理。疟原虫血症水平、存活时间和小鼠体重变化用于确定提取物的抗疟活性。
结果
与阴性对照相比，黑芥子种子提取物产生的化学抑制活性分别为21.88%、50.00%（P<0.01）和53.13%（P<0.01），而在100、200和400mg/kg提取物时，化学预防活性分别为17.42%、21.21%和53.79%（P<0.05）。在4天的抑制试验中，与阴性对照相比，用200和400mg/kg提取物处理的小鼠存活时间显著延长（P<0.05）且体重增加。
结论
从本研究可以得出结论，黑芥子种子提取物显示出良好的化学抑制活性和中等的化学预防活性，该植物可能含有与疟疾治疗和预防相关的生物活性成分，从而支持对该植物活性成分的进一步研究。
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