一种基于罗丹明-冠醚共轭物的新型Cr(3+)荧光探针及其在饮用水检测和生物成像中的应用。
A novel fluorescent probe for Cr(3+) based on rhodamine-crown ether conjugate and its application to drinking water examination and bioimaging.
作者信息
Diao Quanping, Ma Pinyi, Lv Linlin, Li Tiechun, Wang Xinghua, Song Daqian
机构信息
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China; School of Chemistry and Life Science, Anshan Normal University, Ping'an Street 43, Anshan 114005, China.
College of Chemistry, Jilin University, Qianjin Street 2699, Changchun 130012, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Mar 5;156:15-21. doi: 10.1016/j.saa.2015.11.025. Epub 2015 Nov 28.
A trivalent chromium (Cr(3+)) fluorescence probe (RhC) was designed and synthesized via Schiff base reaction based on rhodamine-crown ether conjugate. This probe displayed a favorable selectivity for Cr(3+) over a range of other common metal ions in DMF/H2O (3:7, v/v; PBS buffer 50 mmol L(-1); pH=6.8) solution, leading to prominent fluorescence "OFF-ON" switching of the rhodamine fluorophore. The limit of detection was calculated to be 1.5 μmol L(-1) (S/N=3). The binding ratio of RhC-Cr(3+) complex was determined to be 1:2 according to the Job's plot and HR-MS. The probe was successfully applied to examination of Cr(3+) in drinking water spiked samples. The average recoveries ranged from 104.9% to 106.9% at spiked concentration level of 10.00 μmol L(-1), and the obtained results were consistent with those obtained using atomic absorption spectrometry (AAS). Moreover, bioimaging experiments showed that RhC can sense the Cr(3+) in living cells with a fluorescence enhancement signal.
基于罗丹明-冠醚共轭物，通过席夫碱反应设计并合成了一种三价铬（Cr(3+)）荧光探针（RhC）。在DMF/H2O（3:7，v/v；50 mmol L(-1) PBS缓冲液；pH = 6.8）溶液中，该探针对Cr(3+)表现出优于一系列其他常见金属离子的选择性，导致罗丹明荧光团出现显著的荧光“关-开”切换。计算得出检测限为1.5 μmol L(-1)（S/N = 3）。根据Job曲线和高分辨质谱（HR-MS），确定RhC-Cr(3+)配合物的结合比为1:2。该探针成功应用于加标饮用水样品中Cr(3+)的检测。在加标浓度为10.00 μmol L(-1)时，平均回收率在104.9%至106.9%之间，所得结果与使用原子吸收光谱法（AAS）获得的结果一致。此外，生物成像实验表明，RhC能够通过荧光增强信号检测活细胞中的Cr(3+)。
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