体细胞生成 iPS 细胞中的表观遗传重编程路线图。

The Epigenetic Reprogramming Roadmap in Generation of iPSCs from Somatic Cells.

机构信息

Department of Biomedicine, Aarhus University, Aarhus C 8000, Denmark.

出版信息

J Genet Genomics. 2015 Dec 20;42(12):661-70. doi: 10.1016/j.jgg.2015.10.001. Epub 2015 Oct 23.

DOI:10.1016/j.jgg.2015.10.001

Abstract

Reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is a comprehensive epigenetic process involving genome-wide modifications of histones and DNA methylation. This process is often incomplete, which subsequently affects iPSC reprogramming, pluripotency, and differentiation capacity. Here, we review the epigenetic changes with a focus on histone modification (methylation and acetylation) and DNA modification (methylation) during iPSC induction. We look at changes in specific epigenetic signatures, aberrations and epigenetic memory during reprogramming and small molecules influencing the epigenetic reprogramming of somatic cells. Finally, we discuss how to improve iPSC generation and pluripotency through epigenetic manipulations.

摘要

体细胞重编程为诱导多能干细胞（iPSCs）是一个涉及组蛋白和 DNA 甲基化全基因组修饰的综合表观遗传过程。该过程往往是不完全的，随后会影响 iPSC 重编程、多能性和分化能力。在这里，我们重点回顾 iPSC 诱导过程中组蛋白修饰（甲基化和乙酰化）和 DNA 修饰（甲基化）的表观遗传变化。我们研究了在重编程过程中特定表观遗传特征、异常和表观遗传记忆的变化，以及影响体细胞表观遗传重编程的小分子。最后，我们讨论了如何通过表观遗传操作来提高 iPSC 的生成和多能性。