一种用于比率检测癌细胞内源性二氧化硫衍生物的线粒体靶向荧光探针。
A mitochondria-targeted fluorescent probe for ratiometric detection of endogenous sulfur dioxide derivatives in cancer cells.
作者信息
Li Dong-Peng, Wang Zhao-Yang, Cao Xiang-Jian, Cui Jie, Wang Xin, Cui Hao-Zhong, Miao Jun-Ying, Zhao Bao-Xiang
机构信息
Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, P. R. China.
Institute of Developmental Biology, School of Life Science, Shandong University, Jinan 250100, P. R. China.
出版信息
Chem Commun (Camb). 2016 Feb 14;52(13):2760-3. doi: 10.1039/c5cc09092j.
A new mitochondria-targeted fluorescent probe HCy-D, constructed by dansyl and hemicyanine fluorophores, for SO2 derivatives (HSO3(-)/SO3(2-)) was presented. This probe was designed based on a new FRET platform. HCy-D showed a ratiometric, sensitive and rapid response to HSO3(-)/SO3(2-). Importantly, HCy-D was successfully used for fluorescence imaging of endogenous bisulfite in HepG2 cells, which may benefit cancer diagnosis by discriminating liver cancer cells from normal liver cells.
介绍了一种由丹磺酰基和半花菁荧光团构建的用于检测二氧化硫衍生物（HSO3(-)/SO3(2-)）的新型线粒体靶向荧光探针HCy-D。该探针基于一种新的荧光共振能量转移（FRET）平台设计。HCy-D对HSO3(-)/SO3(2-)表现出比率型、灵敏且快速的响应。重要的是，HCy-D成功用于HepG2细胞内源性亚硫酸氢盐的荧光成像，这可能通过区分肝癌细胞和正常肝细胞而有助于癌症诊断。
Zotero 插件