Skowyra Monika, Gallego Maria Gabriela, Segovia Francisco, Almajano Maria Pilar
Chemical Engineering Department, Technical University of Catalonia, Av. Diagonal, 649, Barcelona 08034, Spain.
Antioxidants (Basel). 2014 Mar 3;3(1):116-28. doi: 10.3390/antiox3010116.
Artemisia annua is currently the only commercial source of the sesquiterpene lactone artemisinin. Although artemisinin is a major bioactive component present in this Chinese herb, leaf flavonoids have shown a variety of biological activities. The polyphenolic profile of extract from leaves of A. annua was assessed as a source of natural antioxidants. Total phenolic content and total flavonoid content were established and three assays were used to measure the antioxidant capacity of the plant extract. The measurement of scavenging capacity against the 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation, the oxygen radical absorbance capacity (ORAC) and the ferric reducing antioxidant power (FRAP) were 314.99 µM Trolox equivalents (TE)/g DW, 736.26 µM TE/g DW and 212.18 µM TE/g DW, respectively. A. annua extracts also showed good antioxidant properties in 10% sunflower oil-in-water emulsions during prolonged storage (45 days) at 32 °C. Artemisia extract at 2 g/L was as effective as butylated hydroxyanisole (BHA) at 0.02 g/L in slowing down the formation of hydroperoxides as measured by peroxide value and thiobarbituric acid reactive substances. The results of this study indicate that extract of A. annua may be suitable for use in the food matrix as substitutes for synthetic antioxidants.
青蒿是目前倍半萜内酯青蒿素的唯一商业来源。尽管青蒿素是这种中草药中的主要生物活性成分,但叶片类黄酮已显示出多种生物活性。对青蒿叶片提取物的多酚谱进行了评估,以确定其作为天然抗氧化剂的来源。测定了总酚含量和总黄酮含量,并使用三种测定方法来测量植物提取物的抗氧化能力。对2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基阳离子的清除能力、氧自由基吸收能力(ORAC)和铁还原抗氧化能力(FRAP)的测定结果分别为314.99 μM Trolox当量(TE)/g干重、736.26 μM TE/g干重和212.18 μM TE/g干重。在32℃下长时间储存(45天)期间,青蒿提取物在10%水包油型向日葵油乳液中也显示出良好的抗氧化性能。以过氧化值和硫代巴比妥酸反应性物质衡量,2 g/L的青蒿提取物在减缓氢过氧化物形成方面与0.02 g/L的丁基羟基茴香醚(BHA)效果相当。本研究结果表明,青蒿提取物可能适合在食品基质中用作合成抗氧化剂的替代品。