翻译后修饰对内在无序蛋白质功能的调控
Modulation of Intrinsically Disordered Protein Function by Post-translational Modifications.
作者信息
Bah Alaji, Forman-Kay Julie D
机构信息
From the Program in Molecular Structure & Function, The Hospital for Sick Children, Toronto, Ontario M5G 0A4 and the Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
出版信息
J Biol Chem. 2016 Mar 25;291(13):6696-705. doi: 10.1074/jbc.R115.695056. Epub 2016 Feb 5.
Abstract
Post-translational modifications (PTMs) produce significant changes in the structural properties of intrinsically disordered proteins (IDPs) by affecting their energy landscapes. PTMs can induce a range of effects, from local stabilization or destabilization of transient secondary structure to global disorder-to-order transitions, potentially driving complete state changes between intrinsically disordered and folded states or dispersed monomeric and phase-separated states. Here, we discuss diverse biological processes that are dependent on PTM regulation of IDPs. We also present recent tools for generating homogenously modified IDPs for studies of PTM-mediated IDP regulatory mechanisms.
摘要
翻译后修饰（PTM）通过影响内在无序蛋白（IDP）的能量景观，使其结构特性发生显著变化。PTM可以引发一系列效应，从局部稳定或破坏瞬时二级结构，到整体的无序到有序转变，有可能驱动内在无序状态与折叠状态之间，或分散单体状态与相分离状态之间的完全状态变化。在这里，我们讨论了依赖于IDP的PTM调控的各种生物过程。我们还介绍了用于生成均匀修饰的IDP以研究PTM介导的IDP调控机制的最新工具。
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