暴露于900兆赫手机辐射对大鼠肝功能的影响。

Ma Hui-rong, Ma Zhi-hong, Wang Gui-ying, Song Cui-miao, Ma Xue-lian, Cao Xiao-hui, Zhang Guo-hong

Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2015 Nov;31(6):567-71.

OBJECTIVE

To study the impacts of exposure to electromagnetic radiation (EMR) on liver function in rats.

METHODS

Twenty adult male Sprague-Dawley rats were randomly divided into normal group and radiated group. The rats in normal group were not radiated, those in radiated group were exposed to EMR 4 h/ d for 18 consecutive days. Rats were sacrificed immediately after the end of the experiment. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and those of malondialdehyde (MDA) and glutathione (GSH) in liver tissue were evaluated by colorimetric method. The liver histopathological changes were observed by hematoxylin and eosin staining and the protein expression of bax and bcl- 2 in liver tissue were detected by immunohistochemical method. Terminal-deoxynucleotidyl transferase mediated nick and labelling (TUNEL) method was used for analysis of apoptosis in liver.

RESULTS

Compared with the normal rats, the serum levels of ALT and AST in the radiated group had no obvious changes (P>0.05), while the contents of MDA increased (P < 0.01) and those of GSH decreased (P < 0.01) in liver tissues. The histopathology examination showed diffuse hepatocyte swelling and vacuolation, small pieces and focal necrosis. The immunohistochemical results displayed that the expression of the bax protein was higher and that of bcl-2 protein was lower in radiated group. The hepatocyte apoptosis rates in radiated group was higher than that in normal group (all P < 0.01).

CONCLUSION

The exposure to 900 MHz mobile phone 4 h/d for 18 days could induce the liver histological changes, which may be partly due to the apoptosis and oxidative stress induced in liver tissue by electromagnetic radiation.

目的

研究暴露于电磁辐射（EMR）对大鼠肝功能的影响。

方法

将20只成年雄性Sprague-Dawley大鼠随机分为正常组和辐射组。正常组大鼠不进行辐射，辐射组大鼠连续18天每天接受4小时的EMR暴露。实验结束后立即处死大鼠。采用比色法评估血清丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平，以及肝组织中丙二醛（MDA）和谷胱甘肽（GSH）水平。通过苏木精-伊红染色观察肝脏组织病理学变化，采用免疫组织化学方法检测肝组织中bax和bcl-2的蛋白表达。采用末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）法分析肝脏细胞凋亡情况。

结果

与正常大鼠相比，辐射组大鼠血清ALT和AST水平无明显变化（P>0.05），而肝组织中MDA含量增加（P<0.01），GSH含量降低（P<0.01）。组织病理学检查显示肝细胞弥漫性肿胀和空泡化，小片和局灶性坏死。免疫组织化学结果显示，辐射组bax蛋白表达较高，bcl-2蛋白表达较低。辐射组肝细胞凋亡率高于正常组（均P<0.01）。