对来自中国一只黑猩猩的非人灵长类动物粪便样本进行的病毒宏基因组学研究发现了人星状病毒。
Viral metagenomics of fecal samples from non-human primates revealed human astrovirus in a chimpanzee, China.
作者信息
Wang Xiaochun, Wang Jinxin, Zhou Chenglin, Yang Shixing, Shen Quan, Zhang Wen, Qi Dunwu
机构信息
Department of Pathogenic Biology, School of Medicine, Jiangsu University, Zhenjiang, 212013 Jiangsu China.
Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000 Sichuan China.
出版信息
Gut Pathog. 2016 Nov 8;8:53. doi: 10.1186/s13099-016-0140-2. eCollection 2016.
BACKGROUND
Human astroviruses (HAstVs) are commonly identified worldwide as important aetiological agents of acute gastroenteritis in all age groups. More and more evidences challenged the paradigm that AstV infections are species-specific. Yet to date, AstVs associated with human infections have not been detected in any animal hosts.
RESULTS
Viral metagenomics methods were used to detect viral nucleic acids in fecal samples from 69 captive non-human primates (NHPs) from three zoos in China. Sequence reads showing high similarity to astrovirus MLB2 were found in feces from a chimpanzee with diarrhea. The complete genome of this astrovirus was determined and deposited in the GenBank under accession number KX273058 (named SAstV-nj). Phylogenetic analysis based on complete genomes revealed that SAstV-nj was closely related to and shared >98% nucleotide sequence identity with the previous human astrovirus MLB2 strains.
CONCLUSIONS
This study suggested that MLB2-related astroviruses might have the potential of cross-species transmission between human and NHP.
背景
人类星状病毒（HAstVs）在全球范围内普遍被认为是各年龄组急性胃肠炎的重要病原体。越来越多的证据对星状病毒感染具有物种特异性这一范式提出了挑战。然而迄今为止，尚未在任何动物宿主中检测到与人类感染相关的星状病毒。
结果
采用病毒宏基因组学方法检测了来自中国三个动物园的69只圈养非人灵长类动物（NHPs）粪便样本中的病毒核酸。在一只腹泻黑猩猩的粪便中发现了与星状病毒MLB2高度相似的序列读数。确定了该星状病毒的完整基因组，并以登录号KX273058保存在GenBank中（命名为SAstV-nj）。基于完整基因组的系统发育分析表明，SAstV-nj与先前的人类星状病毒MLB2毒株密切相关，核苷酸序列同一性大于98%。
结论
本研究表明，与MLB2相关的星状病毒可能具有在人类和非人灵长类动物之间跨物种传播的潜力。
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