嗅鞘细胞与α-晶状体蛋白对成年大鼠视神经损伤修复的协同作用。
Synergistic effect of olfactory ensheathing cells and alpha-crystallin on restoration of adult rat optic nerve injury.
作者信息
Wang Yan Hua, Yin Zheng Qin, Wang Yi
机构信息
Department of Ophthalmology, General Hospital of Chinese People's Liberation Army, Beijing 100853, PR China; Taiyuan Aier Eye Hospital, Aier Eye Hospital Group, Taiyuan 030000, PR China.
Department of Ophthalmology, General Hospital of Chinese People's Liberation Army, Beijing 100853, PR China; Southwest Hospital, Southwest Eye Hospital, Third Military Medical University, Chongqing 400038, PR China.
出版信息
Neurosci Lett. 2017 Jan 18;638:167-174. doi: 10.1016/j.neulet.2016.12.042. Epub 2016 Dec 19.
Olfactory enshesathing cells (OECs) and α-crystallin all can promote axonal regeneration after optic nerve injury. However, their mechanisms were different. Here, we study the synergistic effect of OECs and α-crystallin on the optic nerve regeneration. α-Crystallin was injected into vitreous cavity, and OECs were transplanted to the optic nerve injury area. The regeneration length of optic nerve were measured by anterograde tracing using cholera toxin subunit B (CTB). The survival of RGCs were assessed by counting the numbers of βIII-tubulin-labeled RGCs in a retinal whole mount. The results that OECs and α-crystallin all could promoted RGCs survival and axonal regeneration (P<0.01). Especially in the combination group, the length of axonal regeneration was 5.6mm after optic nerve injury for 3 months. These findings indicated that compared to OECs and α-crystallin alone, the combination of OECs and α-crystallin could promote axonal regeneration more effectively.
嗅鞘细胞（OECs）和α-晶状体蛋白均能促进视神经损伤后的轴突再生。然而，它们的机制有所不同。在此，我们研究OECs和α-晶状体蛋白对视神经再生的协同作用。将α-晶状体蛋白注入玻璃体腔，并将OECs移植到视神经损伤区域。使用霍乱毒素B亚基（CTB）通过顺行示踪法测量视神经的再生长度。通过计数视网膜整装片中βIII-微管蛋白标记的视网膜神经节细胞（RGCs）数量来评估RGCs的存活情况。结果表明，OECs和α-晶状体蛋白均能促进RGCs存活和轴突再生（P<0.01）。特别是在联合组中，视神经损伤3个月后轴突再生长度为5.6毫米。这些发现表明，与单独使用OECs和α-晶状体蛋白相比，OECs与α-晶状体蛋白联合使用能更有效地促进轴突再生。
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