氨基酸功能化的银纳米粒子修饰在氧化石墨烯片上的抗菌性能。
Antibacterial properties of amino acid functionalized silver nanoparticles decorated on graphene oxide sheets.
机构信息
School of Studies in Chemistry, Pt. Ravishankar Shukla University, Raipur 492010, (C.G.), India.
Department of Chemistry, Govt. Madhav Science P. G. College, Ujjain 456010, (M.P.), India.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jun 15;181:47-54. doi: 10.1016/j.saa.2017.03.032. Epub 2017 Mar 16.
Graphene oxide (GO) sheets decorated with amino acid L-cysteine (L-cys) functionalized silver nanoparticles (GO-L-cys-Ag) was synthesized by AgNO, trisodium citrate, and NaBH. GO-L-cys-Ag nanocomposite was characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectra, ultraviolet-visible (UV-vis) absorption spectra, which demonstrated that a diameter of L-cys-AgNPs compactly deposited on GO. Antibacterial activity tests of GO-L-cys-Ag nanocomposite were carried out using Escherichia coli MTCC 1687 and Staphylococcus aureus MTCC 3160 as model strains of Gram-negative and Gram-positive bacteria, respectively. The effect of bactericide dosage on antibacterial activity of GO-L-cys-Ag nanocomposite was examined by plate count, well diffusion and broth dilution methods. Morphological observation of bacterial cells by scanning electron microscope (SEM) showed that GO-L-cys-Ag nanocomposite was more destructive to cell membrane of Escherichia coli than that of Staphylococcus aureus. The above technique establish that the bactericidal property of GO-L-cys-Ag nanocomposite with wide range of applications in biomedical science.
氧化石墨烯（GO）片上装饰有氨基酸 L-半胱氨酸（L-cys）功能化的银纳米粒子（GO-L-cys-Ag），通过 AgNO、柠檬酸钠和 NaBH 合成。GO-L-cys-Ag 纳米复合材料通过透射电子显微镜（TEM）、傅里叶变换红外（FTIR）光谱、紫外-可见（UV-vis）吸收光谱进行了表征，证明 L-cys-AgNPs 的直径紧密沉积在 GO 上。使用大肠杆菌 MTCC 1687 和金黄色葡萄球菌 MTCC 3160 作为革兰氏阴性菌和革兰氏阳性菌的模型菌株，进行了 GO-L-cys-Ag 纳米复合材料的抗菌活性测试。通过平板计数、孔扩散和肉汤稀释法检查了杀菌剂剂量对 GO-L-cys-Ag 纳米复合材料抗菌活性的影响。扫描电子显微镜（SEM）观察细菌细胞的形态显示，GO-L-cys-Ag 纳米复合材料对大肠杆菌细胞膜的破坏作用大于对金黄色葡萄球菌的破坏作用。上述技术确立了 GO-L-cys-Ag 纳米复合材料具有广泛的应用前景，可用于生物医学科学。
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