一种新型的“turn-on”硫代氧杂荧光素比色和荧光传感器，用于 Hg 及其在活细胞中的应用。
A novel "turn-on" thiooxofluorescein-based colorimetric and fluorescent sensor for Hg and its application in living cells.
机构信息
College of Chemistry, Jilin University, Changchun 130021, PR China; College of Materials Science and Engineering, Jilin University of Chemical Technology, Jilin 132022, PR China.
National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130021, PR China.
出版信息
Talanta. 2017 Aug 1;170:103-110. doi: 10.1016/j.talanta.2017.03.099. Epub 2017 Mar 31.
A novel water-soluble fluorescent probe FLS2 based on the thiooxofluorescein derivative has been firstly designed and synthesized. UV-vis absorption and fluorescence spectra studies showed that the FLS2 as a colorimetric and ratiometric fluorescent probe exhibited high selectivity and sensitivity towards Hg, which was mainly attributed to the special binding with the receptor unit accompanied with the spirolactam ring-opening progress. In addition, the probe FLS2 could be used as a naked-eye indicator for Hg with reversible response. It displayed approximate 37-fold fluorescent enhancement at 529nm in the presence of only 2.0 equiv. Hg and the detection limit was calculated at about 39nM. What's more, cellular imaging experiment revealed that the sensor had excellent biocompatibility and low cytotoxicity that could be utilized for monitoring Hg in living cells.
一种基于硫代氧杂荧光素衍生物的新型水溶性荧光探针 FLS2 已被首次设计和合成。紫外-可见吸收和荧光光谱研究表明，FLS2 作为一种比色和比率荧光探针，对 Hg 具有高选择性和灵敏度，这主要归因于与受体单元的特殊结合，伴随着螺环酰胺开环过程。此外，探针 FLS2 可以用作 Hg 的肉眼指示剂，具有可逆响应。在仅存在 2.0 当量 Hg 的情况下，在 529nm 处显示出约 37 倍的荧光增强，检测限约为 39nM。更重要的是，细胞成像实验表明，该传感器具有优异的生物相容性和低细胞毒性，可用于监测活细胞中的 Hg。
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