对 PET 水解酶催化机制的结构洞察。
Structural insight into catalytic mechanism of PET hydrolase.
机构信息
Industrial Enzymes National Engineering Laboratory, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
AsiaPac Biotechnology Co., Ltd, Dongguan, 523808, China.
出版信息
Nat Commun. 2017 Dec 13;8(1):2106. doi: 10.1038/s41467-017-02255-z.
PET hydrolase (PETase), which hydrolyzes polyethylene terephthalate (PET) into soluble building blocks, provides an attractive avenue for the bioconversion of plastics. Here we present the structures of a novel PETase from the PET-consuming microbe Ideonella sakaiensis in complex with substrate and product analogs. Through structural analyses, mutagenesis, and activity measurements, a substrate-binding mode is proposed, and several features critical for catalysis are elucidated.
聚对苯二甲酸乙二醇酯水解酶（PETase）能够将聚对苯二甲酸乙二醇酯（PET）水解成可溶性单体，为塑料的生物转化提供了一条有吸引力的途径。在这里，我们展示了一种新型 PETase 的结构，该酶来自于消耗 PET 的微生物 Ideonella sakaiensis，与底物和产物类似物形成复合物。通过结构分析、突变和活性测量，提出了一种底物结合模式，并阐明了几个对催化至关重要的特征。
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