TAT-MyoD融合蛋白的综述：现状与待解决的问题
A mini-review of TAT-MyoD fused proteins: state of the art and problems to solve.
作者信息
Patruno Marco, Melotti Luca, Gomiero Chiara, Sacchetto Roberta, Topel Ohad, Martinello Tiziana
机构信息
Department of Comparative Biomedicine and Food Science, University of Padova, Italy.
VTH - Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Israel.
出版信息
Eur J Transl Myol. 2017 Dec 5;27(4):6039. doi: 10.4081/ejtm.2017.6039.
The transcriptional activator TAT is a small peptide essential for viral replication and possesses the property of entering the cells from the extracellular milieu, acting as a membrane shuttle. In order to safely differentiate cells an innovative methodology, based on the fusion of transcription factors and the TAT sequence, is discussed in this short review. In several studies, it has been demonstrated that TAT protein can be observed in the cell nucleus after few hours from the inoculation although its way of action is not fully understood. However, further studies will be necessary to develop this methodology for clinical purposes.
转录激活因子TAT是一种对病毒复制至关重要的小肽，具有从细胞外环境进入细胞的特性，可作为膜穿梭载体。为了安全地诱导细胞分化，本简短综述讨论了一种基于转录因子与TAT序列融合的创新方法。在多项研究中，已证明接种后数小时即可在细胞核中观察到TAT蛋白，但其作用方式尚未完全明确。然而，为了临床应用开发该方法还需要进一步研究。
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