一种用于检测铜的罗丹明-三唑荧光化学传感器及其在生物成像中的应用。
A rhodamine-triazole fluorescent chemodosimeter for Cu detection and its application in bioimaging.
作者信息
Wechakorn Kanokorn, Prabpai Samran, Suksen Kanoknetr, Kanjanasirirat Phongthon, Pewkliang Yongyut, Borwornpinyo Suparerk, Kongsaeree Palangpon
机构信息
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Center for Excellence in Protein and Enzyme Technology, Faculty of Science, Mahidol University, Bangkok, Thailand.
出版信息
Luminescence. 2018 Feb;33(1):64-70. doi: 10.1002/bio.3373. Epub 2017 Jul 17.
A rhodamine-based fluorescent chemodosimeter rhodamine hydrazide-triazole (RHT) tethered with a triazole moiety was developed for Cu detection. In aqueous medium, the RHT probe exhibited high selectivity and sensitivity toward Cu among other metal ions. The addition of Cu triggered a fluorescence emission of RHT by 384-fold (Φ = 0.33) based on a ring-opening process and a subsequent hydrolysis reaction. Moreover, RHT also showed a selective colorimetric response toward Cu from colorless solution to pink, readily observed with the naked eye. The limit of detection of RHT for Cu was calculated to be 1 nM (0.06 ppb). RHT was successfully demonstrated to detect Cu in Chang liver cells by confocal fluorescence microscopy.
开发了一种基于罗丹明的荧光化学传感器——罗丹明酰肼 - 三唑（RHT），其与三唑部分相连，用于检测铜。在水性介质中，RHT探针在其他金属离子中对铜表现出高选择性和灵敏度。加入铜会基于开环过程和随后的水解反应使RHT的荧光发射增强384倍（Φ = 0.33）。此外，RHT对铜还表现出从无色溶液到粉红色的选择性比色响应，肉眼即可轻松观察到。RHT对铜的检测限经计算为1 nM（0.06 ppb）。通过共聚焦荧光显微镜成功证明RHT可检测Chang肝细胞中的铜。
Zotero 插件