利用化学探针检测马来酸诱导的肾小管损伤的蛋白质组学。

The use of chemical probes to detect the proteomics of renal tubular injury induced by maleic acid.

机构信息

Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Nephrology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Physiology & Biophysics, University of California at Irvine, Irvine, CA 92697, USA; UC Irvine Diabetes Center, University of California at Irvine, Irvine, CA 92697, USA.

Department of Medical Science Industry, College of Health Sciences, Chang Jung Christian University, Taiwan.

出版信息

J Chromatogr A. 2018 Aug 31;1565:96-104. doi: 10.1016/j.chroma.2018.06.040. Epub 2018 Jun 19.

DOI:10.1016/j.chroma.2018.06.040

PMID:29983170

Abstract

Maleic acid (MA), an industrial raw material, was found to be illegally added to edible starch-based food products in Taiwan in 2013, a practice unheard of in most of the world. MA has been associated with renal dysfunction in many experimental animal studies. In this study, we developed chemical probes to investigate protein-protein interactions between MA and renal proteins. In the fabrication of the MA probes, we used silicon dioxide (SiO) modified with a silanized linker (3-aminopropyl triethoxyslane, APTES) to generate MA with APTES-SiO particles. The probes were then incubated with the cell lysates of normal human kidney cell lines (HK-2) and subjected to MS/MS for identifying several MA-related proteins, including nucleophosmin, neutral alpha-glucosidase AB, translocon-associated protein subunit alpha, elongation factor 1-gamma, 60S acidic ribosomal protein P0-like, and heat shock protein (HSP 90-alpha and beta). Based on our findings, we believed that the probe can potentially be used to identify and detect the target proteins and help characterize a network of MA protein-protein interactions.

摘要

2013 年，台湾发现有人将工业原料马来酸非法添加到食用淀粉类食品中，这种做法在世界大部分地区闻所未闻。许多实验动物研究表明，马来酸与肾功能障碍有关。在这项研究中，我们开发了化学探针来研究马来酸与肾脏蛋白之间的蛋白质-蛋白质相互作用。在 MA 探针的制备中，我们使用硅烷化接头（3-氨丙基三乙氧基硅烷，APTES）修饰的二氧化硅（SiO）生成 APTES-SiO 颗粒与马来酸。然后将探针与正常人肾细胞系（HK-2）的细胞裂解物一起孵育，并进行 MS/MS 以鉴定几种与马来酸相关的蛋白质，包括核磷蛋白、中性α-葡糖苷酶 AB、易位子相关蛋白亚基α、延伸因子 1-γ、60S 酸性核糖体蛋白 P0 样和热休克蛋白（HSP 90-α和β）。根据我们的发现，我们相信该探针有可能被用于鉴定和检测靶蛋白，并有助于描述马来酸蛋白质-蛋白质相互作用网络。