Highly Stable and NIR Luminescent Ru-LPMSN Hybrid Materials for Sensitive Detection of Cu in Vivo.

Herein, new near-infrared (NIR) luminescent ruthenium complexes were prepared for detecting Cu ions. Then, ruthenium complex hybrid nanomaterials (Ru-LPMSNs) were fabricated successfully by imbedding the ruthenium complex into mesoporous silica nanoparticles. Benefiting from the novel large-pore mesoporous structure and good adsorbility of LPMSNs, Ru-LPMSN hybrid materials showed a significantly enhanced fluorescence intensity and stability. NIR fluorescence of Ru-LPMSNs was rapidly quenched by Cu ions. Ru-LPMSNs also showed high Cu ion selectivity and sensitivity as a sensor. The detection limit of Cu ions was 10 nM with a wide linear relationship between the fluorescence intensity of Ru-LPMSNs and the concentration of Cu ions. The mechanism of fluorescence quenching might be that the combination of the ruthenium complex and Cu ions constrained the photoinduced electron-transfer process. Furthermore, Ru-LPMSNs dramatically increased the fluorescence signals in cells and achieved Cu-ion detection. Ru-LPMSNs had different tissue affinities and could monitor distribution of exogenous Cu ions in vivo. Moreover, Ru-LPMSNs realized direct and rapid detection of Cu-ion content in serum. These results indicated the potential applications of the prepared nanomaterials as Cu detection agents.