Comparison of the Vitek MS and Bruker Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry Systems for Identification of Chryseobacterium Isolates from Clinical Specimens and Report of Uncommon Chryseobacterium Infections in Humans.

Matrix-assisted laser desorption ionization-time of flight mass spectrometry is becoming more popular and is replacing traditional identification methods in the clinical microbiology laboratory. We aimed to compare the Vitek mass spectrometry (MS) and Bruker Biotyper systems for the identification of isolated from clinical specimens and to report uncommon infections in humans. The microbial database from a hospital was searched for records between 2005 and 2016 to identify cultures that yielded Species identification by the Vitek MS and Bruker Biotyper systems was compared to identification by 16S rRNA gene sequencing. Over the study period, 140 isolates were included. Based on 16S rRNA gene sequencing, 78 isolates were , 39 were , 12 were uncommon species (, , , , , and ), and the remaining 11 isolates were only identified at the genus level. The Vitek MS and Bruker Biotyper systems correctly identified 98.7% and 100% of isolates, respectively. While the Bruker Biotyper accurately identified 100% of isolates, the Vitek MS system correctly identified only 2.6% of isolates from this species. None of the uncommon species were successfully identified by either of these two systems. The overall accuracies of identification at the species level by the Vitek MS and Bruker Biotyper systems were 60.5% and 90.7%, respectively. An upgrade and correction of the Vitek MS and Bruker Biotyper databases is recommended to correctly identify species.