基于酚酞的荧光探针用于在水相介质和活细胞中顺序检测 Al 和 F 离子。
A phenolphthalein-based fluorescent probe for the sequential sensing of Al and F ions in aqueous medium and live cells.
机构信息
School of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
School of Chemistry and Chemical Engineering, Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Feb 5;208:131-139. doi: 10.1016/j.saa.2018.09.064. Epub 2018 Oct 6.
A novel fluorescent probe, phenolphthalein‑dialdehyde‑(2‑pyridyl) hydrazone (L), for sequentially detecting Al and F in almost 100% aqueous medium was successfully designed and synthesized. The probe offers two binding pockets for Al to form a 1: 2 ligand/metal complex, leading to a significant fluorescence enhancement at 465 nm. Further, the in-situ formed L-Al complex acts as a secondary fluorescent chemosensor for F by quenching the fluorescence of the complex with high selectivity. The detection limit for Al and F sensing is 2.28 nM and 0.13 μM, respectively, which are far below the World Health Organization (WHO) acceptable limits (7.41 μM for Al ion and 79 μM for F) in drinking water. The probe L was successfully applied to the detection of Al and F in cells using fluorescence microscopy.
一种新型荧光探针，酚酞醛-（2-吡啶基）腙（L），成功设计并合成用于在近 100%水介质中顺序检测 Al 和 F。该探针提供了两个结合口袋供 Al 形成 1:2 配体/金属配合物，导致在 465nm 处显著荧光增强。此外，原位形成的 L-Al 配合物通过高选择性猝灭配合物的荧光，充当 F 的二次荧光化学传感器。Al 和 F 感应的检测限分别为 2.28nM 和 0.13μM，远低于世界卫生组织（WHO）在饮用水中可接受的限值（Al 离子为 7.41μM，F 为 79μM）。该探针 L 成功应用于荧光显微镜检测细胞中的 Al 和 F。
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