用于检测 Zn 和 Cu 的荧光比色希夫碱化学传感器：在活细胞成像和比色检测试剂盒中的应用。
A fluorescent and colorimetric Schiff base chemosensor for the detection of Zn and Cu: Application in live cell imaging and colorimetric test kit.
机构信息
Department of Fine Chem., Seoul National Univ. of Sci. and Tech. (SNUT), Seoul 01811, Republic of Korea.
Department of Chem., KAIST, Daejeon 34141, Republic of Korea.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Mar 15;211:34-43. doi: 10.1016/j.saa.2018.11.058. Epub 2018 Nov 23.
A novel Schiff base chemosensor HMID, ((E)‑1‑((2‑hydroxy‑3‑methoxybenzylidene)amino)imidazolidine‑2,4‑dione), have been designed and synthesized. Sensor HMID showed a selectivity to Zn through fluorescence enhancement in aqueous solution. Its detection limit was analyzed as 11.9 μM. Importantly, compound HMID could be applied to image Zn in live cells. Detection mechanism of Zn by HMID was suggested to be an effect of chelation-enhanced fluorescence (CHEF) by DFT calculations. Moreover, HMID could detect Cu with a change of color from colorless to pink. The selective detection mechanism of Cu by HMID was demonstrated to be the promotion of intramolecular charge transfer band by DFT calculations. Additionally, HMID could be employed as a naked-eye colorimetric kit for Cu. Therefore, HMID has the ability as a 'single sensor for dual targets'.
一种新型希夫碱化学传感器 HMID，((E)-1-((2-羟基-3-甲氧基亚苄基)氨基)咪唑烷-2,4-二酮)，已经被设计和合成。传感器 HMID 在水溶液中通过荧光增强显示出对 Zn 的选择性。其检测限分析为 11.9 μM。重要的是，化合物 HMID 可以用于活细胞中 Zn 的成像。通过 DFT 计算，HMID 对 Zn 的检测机制被认为是螯合增强荧光（CHEF）的影响。此外，HMID 可以检测 Cu，颜色从无色变为粉红色。通过 DFT 计算，HMID 对 Cu 的选择性检测机制被证明是促进分子内电荷转移带。此外，HMID 可以用作 Cu 的肉眼比色试剂盒。因此，HMID 具有作为“双靶单传感器”的能力。
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