Characterization a Novel Butyric Acid-Producing Bacterium Subsp. Subsp. Nov.

Butyrate-producing bacteria can biosynthesize butyrate and alleviate inflammatory diseases. However, few studies have reported that the genus has the ability to produce butyric acid. Here, our study depicts a strain, which is a rod-shaped obligate anaerobe that is able to produce butyric acid. This microorganism was isolated from a human gut, and the optimal growth conditions were found to be 37 °C on PYG medium with pH 6.5. The 16S rRNA gene sequence demonstrated that this microorganism shared 99.93% similarity with . ATCC 25986, which was higher than the threshold (98.65%) for differentiating two species. Digital DNA⁻DNA hybridization and average nucleotide identity values also supported that this microorganism belonged to the species . Distinct phenotypic characteristics between TF06-26 and the type strain of . , such as the fermentation of D-lactose, D-fructose and D-maltose, positive growth under pH 5 and 0.2% (/) cholate, suggested this strain was a novel subspecies. Comparative genome analysis revealed that butyric acid kinase and phosphate butyryltransferase enzymes were coded exclusively by this strain, indicating a specific butyric acid-producing function of this . subspecies within the genus . Thus, subsp. subsp. nov. was proposed, with set strain TF06-26 (=CGMCC 1.5216 = DSM 105138) as the type strain.