临床宏基因组学。
Clinical metagenomics.
机构信息
Department of Laboratory Medicine, University of California, San Francisco, CA, USA.
Department of Medicine, Division of Infectious Diseases, University of California, San Francisco, CA, USA.
出版信息
Nat Rev Genet. 2019 Jun;20(6):341-355. doi: 10.1038/s41576-019-0113-7.
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly moving from research to clinical laboratories. This emerging approach is changing how physicians diagnose and treat infectious disease, with applications spanning a wide range of areas, including antimicrobial resistance, the microbiome, human host gene expression (transcriptomics) and oncology. Here, we focus on the challenges of implementing mNGS in the clinical laboratory and address potential solutions for maximizing its impact on patient care and public health.
临床宏基因组下一代测序（mNGS）是对患者样本中的微生物和宿主遗传物质（DNA 和 RNA）进行全面分析，该技术正在迅速从研究实验室进入临床实验室。这种新兴方法正在改变医生诊断和治疗传染病的方式，其应用涵盖了广泛的领域，包括抗菌药物耐药性、微生物组、人类宿主基因表达（转录组学）和肿瘤学。在这里，我们重点讨论在临床实验室中实施 mNGS 所面临的挑战，并探讨了最大限度地发挥其对患者护理和公共卫生影响的潜在解决方案。
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