基于层状钴(II)双氢氧化物纳米片的荧光探针法测定三磷酸腺苷

A fluorometric displacement assay for adenosine triphosphate using layered cobalt(II) double hydroxide nanosheets.

机构信息

CAS Key Laboratory of Design and Assembly of Functional Nanostructures, and Fujian Provincial Key Laboratory of Nanomaterials, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian, 350002, People's Republic of China.

School of Ocean and Biochemical Engineering, Fuqing Branch of Fujian Normal University, Fuqing, Fujian Province, 350300, People's Republic of China.

出版信息

Mikrochim Acta. 2019 Mar 30;186(4):263. doi: 10.1007/s00604-019-3371-3.

DOI:10.1007/s00604-019-3371-3

PMID:30929077

Abstract

A turn-on fluorometric method is described for the determination of adenosine-5'-triphosphate (ATP). It is based on the displacement of a dye-labeled oligonucleotide from a cobalt(II) based layered double hydroxide (LDH). Due to the electrostatic and ligand exchange interaction, the FAM-labeled DNA is readily adsorbed on the LDH. This leads to complete and fast quenching of the green fluorescence of the label. However, on addition of ATP, the DNA is detached from the LDH because of the stronger affinity of ATP for LDH. This results in the restoration of the green fluorescence. The effect was used to design a sensitive assay that has a linear response in the 0.5-100 μM ATP concentration range and a 0.23 μM lower detection limit. It was applied to the determination of ATP in spiked serum samples. Graphical abstract Schematic presentation of a fluorometric ATP assay based on the displacement of a dye-labeled oligonucleotide from a layered double hydroxide (LDH).

摘要

一种荧光开启方法用于测定三磷酸腺苷（ATP）。它基于从基于钴（II）的层状双氢氧化物（LDH）中置换出一种染料标记的寡核苷酸。由于静电和配体交换相互作用，FAM 标记的 DNA 很容易被 LDH 吸附。这导致标签的绿色荧光完全且快速猝灭。然而，加入 ATP 后，由于 ATP 与 LDH 的亲和力更强，DNA 从 LDH 上脱离。这导致绿色荧光恢复。该效应被用于设计一种灵敏的测定方法，该方法在 0.5-100 μM ATP 浓度范围内具有线性响应，检测下限为 0.23 μM。它被应用于测定加标血清样品中的 ATP。