携带大肠杆菌乳糖基因的乳糖培养副溶血性弧菌的耐盐性
Salt tolerance of lactose-grown Vibrio parahaemolyticus carrying Escherichia coli lac genes.
作者信息
Datta A R, MacQuillan A M
出版信息
Appl Environ Microbiol. 1987 Feb;53(2):466-9. doi: 10.1128/aem.53.2.466-469.1987.
Abstract
Lac- strains of Vibrio parahaemolyticus were converted to Lac+ on receiving a hybrid plasmid containing the lactose utilization genes of Escherichia coli K-12. A V. parahaemolyticus strain containing this hybrid plasmid exhibited optimal growth rates on glucose and other carbon sources in the presence of 0.2 to 0.4 M NaCl. Growth of the same strain on lactose was inhibited at similar concentrations of NaCl. The altered growth rate responses in lactose medium appeared to be attributable to effects of NaCl on the activity of lactose permease, and possibly on that of beta-galactosidase, rather than on the levels of these enzymes in V. parahaemolyticus cells.
摘要
副溶血性弧菌的乳糖利用缺陷型菌株在获得含有大肠杆菌K-12乳糖利用基因的杂交质粒后转变为乳糖利用阳性型。含有这种杂交质粒的副溶血性弧菌菌株在0.2至0.4M氯化钠存在的情况下，在葡萄糖和其他碳源上表现出最佳生长速率。同一菌株在乳糖上的生长在相似浓度的氯化钠下受到抑制。乳糖培养基中生长速率的改变似乎归因于氯化钠对乳糖通透酶活性的影响，也可能对β-半乳糖苷酶活性的影响，而不是对副溶血性弧菌细胞中这些酶水平的影响。
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